Over-expression of BcFLC1 from non-heading Chinese cabbage enhances cold tolerance in Arabidopsis

被引:13
作者
Liu, T. [1 ,2 ,3 ]
Li, Y. [1 ,2 ,3 ]
Ren, J. [1 ,2 ,3 ]
Zhang, C. [3 ]
Kong, M. [1 ,2 ,3 ]
Song, X. [1 ,2 ,3 ]
Zhou, J. [1 ,2 ,3 ]
Hou, X. [1 ,2 ,3 ]
机构
[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops E, Nanjing 210095, Jiangsu, Peoples R China
[3] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
关键词
Brassica campestris; electrolyte leakage; flowering; low temperature; malondialdehyde; proline; MADS DOMAIN PROTEIN; LOCUS-C FLC; FLOWERING-TIME; FREEZING TOLERANCE; GENE; VERNALIZATION; CONFERS; STRESS; ANTIOXIDANT; REPRESSOR;
D O I
10.1007/s10535-012-0287-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A gene (named BcFLC1) homologous to the AtFLC gene, which encodes a floral repressor, was isolated from the non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis) cv. NJ074. The gene showed high similarity to AtFLC. For studying the gene function, we designed to introduce the BcFLC1 gene into Arabidopsis thaliana. The results showed that BcFLC1 had effects on flowering time similar to AtFLC. We also found that Arabidopsis cold-tolerance was enhanced by BcFLC1 overexpression. Under low temperature stress, the BcFLC1 transgenic plants exhibited stronger growth than wild-type plants. The elevated cold tolerance of the BcFLC1 over-expressing plants was also confirmed by the changes of electrolyte leakage and malonyldialdehyde and proline content.
引用
收藏
页码:262 / 266
页数:5
相关论文
共 50 条
[21]   BcWRKY33A Enhances Resistance to Botrytis cinerea via Activating BcMYB51-3 in Non-Heading Chinese Cabbage [J].
Wang, Huiyu ;
Zheng, Yushan ;
Xiao, Dong ;
Li, Ying ;
Liu, Tongkun ;
Hou, Xilin .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (15)
[22]   Over-expression of SgRVE1 from fine-stem stylo (Stylosanthes guianensis var. intermedia) up-regulates CBF gene expression and enhances cold tolerance in Arabidopsis [J].
Wang, Cong-Cong ;
Liang, Chen ;
Li, Han-Ying ;
Huang, Huai-An ;
He, Liang-Liang ;
Liu, Cui-Ling ;
Wu, Zhi-Hao ;
Gui, Chun-Sheng ;
Ren, Ci ;
Wang, Yi-Hua ;
Yang, Hao-Yu ;
Zhong, Dan-Wen ;
Peng, Xiao-Qun ;
Fu, Cheng-Cheng ;
Xie, Xin-Ming ;
Chen, Shu .
PLANT CELL TISSUE AND ORGAN CULTURE, 2024, 158 (02)
[23]   Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance [J].
Xue, Y. ;
Peng, R. ;
Xiong, A. ;
Li, X. ;
Zha, D. ;
Yao, Q. .
BIOLOGIA PLANTARUM, 2010, 54 (01) :105-111
[24]   C-repeat binding factor gene family identified in non-heading Chinese cabbage is functional in abiotic and biotic stress response but different from that in Arabidopsis [J].
Wang, Zhen ;
Wang, Feng ;
Tang, Jun ;
Huang, Zhinan ;
Xiong, Aisheng ;
Hou, Xilin .
ACTA PHYSIOLOGIAE PLANTARUM, 2014, 36 (12) :3217-3229
[25]   Over-expression of ZmPti1, a homologue to Pti1, increases salt tolerance of Arabidopsis thaliana [J].
Zou, Huawen ;
Wu, Zhongyi ;
Zhang, Xiuhai ;
Wang, Yongqin ;
Huang, Conglin .
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (05) :656-662
[26]   BcBZR1 Regulates Leaf Inclination Angle in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis Makino) [J].
Lin, Wenyuan ;
Li, Yiran ;
He, Ying ;
Wu, Ying ;
Hou, Xilin .
HORTICULTURAE, 2024, 10 (04)
[27]   Constitutive over-expression of rice ClpD1 protein enhances tolerance to salt and desiccation stresses in transgenic Arabidopsis plants [J].
Mishra, Ratnesh Chandra ;
Richa ;
Grover, Anil .
PLANT SCIENCE, 2016, 250 :69-78
[28]   Over-expression of Arabidopsis ORANGE gene enhances drought stress tolerance through ABA-dependent pathway in Arabidopsis thaliana [J].
Shan, Yong-Jie ;
Li, Dan ;
Cao, Jing-Jing ;
Zhang, Li ;
Han, Li-Quan ;
Zhang, Mei-Ping ;
Shen, Zhen-Guo .
PLANT GROWTH REGULATION, 2022, 96 (01) :91-101
[29]   Regulation of the trade-off between cold stress and growth by glutathione S-transferase phi class 10 (BcGSTF10) in non-heading Chinese cabbage [J].
Shen, Yunlou ;
Wang, Guangpeng ;
Ran, Jiajun ;
Li, Yiran ;
Wang, Huiyu ;
Ding, Qiang ;
Li, Ying ;
Hou, Xilin .
JOURNAL OF EXPERIMENTAL BOTANY, 2024, 75 (07) :1887-1902
[30]   Cloning and Expression Analysis of A Partial bcDREB2 Relative Gene in Non-heading Chinese Cabbage (Brassica campestris ssp chinensis Makino) [J].
Han, Jian-Ming ;
Xu, Bin ;
Li, Xue-Bin ;
Wang, Ying .
ICMS2010: PROCEEDINGS OF THE THIRD INTERNATIONAL CONFERENCE ON MODELLING AND SIMULATION, VOL 4: MODELLING AND SIMULATION IN BIOLOGY, ECOLOGY & ENVIRONMENT, 2010, :112-117