Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fasus sylvatica L. seeds

被引：14

作者：

Angel Jimenez, Jesus ^{[1
]}

Alonso-Ramirez, Ana ^{[1
]}

Nicolas, Carlos ^{[1
]}

机构：

[1] Univ Salamanca, Fac Biol, Ctr Hispano Luso Invest Agr, Dept Fisiol Vegetal, Salamanca 37007, Spain

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 165卷 / 17期

关键词：

Abscisic acid; Caleosin; Dehydrin; Drought responses; Fagus sylvatica;

D O I：

10.1016/j.jplph.2007.11.013

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed. (C) 2008 Elsevier GmbH. Att rights reserved.

引用

页码：1798 / 1807

页数：10

共 45 条

[1] ONSET OF DESICCATION TOLERANCE DURING DEVELOPMENT OF THE BARLEY EMBRYO [J].