Role of protein kinase C in cytokine secretion by lung epithelial cells during infection with Paracoccidioides brasiliensis

被引：9

作者：

Alcantara, Cristiane ^{[1
]}

Maza, Paloma Korehisa ^{[1
]}

Silva, Bianca Carla ^{[1
]}

Barros, Campitelli ^{[1
]}

Suzuki, Erika ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, SP, Brazil

来源：

PATHOGENS AND DISEASE | 2015年 / 73卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

fungi; innate immunity; cell signaling; protein kinase C; cytokine; epithelial cell; PKC-DELTA; ACTIVATION; EXPRESSION; ISOFORMS; RECEPTOR; INNATE; LEADS; IL-8;

D O I：

10.1093/femspd/ftv045

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we investigated the role of protein kinases C (PKCs) in interleukin (IL)-6 and IL-8 secretion by human lung epithelial A549 cells during infection with the fungal pathogen Paracoccidioides brasiliensis. Rottlerin and the broad spectrum PKC inhibitor Go 6983 reduced cytokine levels in A549 cell-P. brasiliensis cultures. Next, by western blot, we verified that infection with this fungus led to phosphorylation of PKC delta (Thr(505)). By using a peptide inhibitor for PKC delta or PKC delta short interfering RNA technique, IL-6 and IL-8 levels in A549-P. brasiliensis cultures were also reduced. Together, these results indicate that P. brasiliensis promotes IL-6 and IL-8 secretion by A549 cells in a PKC delta-dependent manner.

引用

页数：7

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