Human Elastin-Based Recombinant Biopolymers Improve Mesenchymal Stem Cell Differentiation

被引：25

作者：

Celebi, Betuel ^{[2
]}

Cloutier, Maxime ^{[2
]}

Balloni, Rodrigo ^{[2
]}

Mantovani, Diego ^{[2
]}

Bandiera, Antonella ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Vita, I-34127 Trieste, Italy

[2] Univ Laval, Lab Biomat & Bioengn, Quebec City, PQ G1V 0A6, Canada

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 11期

基金：

加拿大自然科学与工程研究理事会;

关键词：

biomimetic polypeptides; bone-marrow mesenchymal stem cells; collagen type I; elastin; osteogenic differentiation; EXTRACELLULAR-MATRIX; OSTEOGENIC-DIFFERENTIATION; EXPRESSION; CULTURE; BIOMATERIALS; POLYPEPTIDES; POLYMERS; INTEGRIN; BEHAVIOR; ERK;

D O I：

10.1002/mabi.201200170

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Elastin-based polypeptides are a class of smart biopolymers representing an important model in the design of biomaterials. The combination of biomimetic materials with cells that have great plasticity provides a promising strategy for the realization of highly engineered cell-based constructs for regenerative medicine and tissue repair applications. Two recombinant biopolymers inspired by human elastin are assessed as coating agents to prepare biomimetic surfaces for cell culture. These substrates are assayed for hBM MSC culture. The coated surfaces are also characterized with AFM to evaluate the topographical features of the deposited biopolymers. The results suggest that the elastin-derived biomimetic surfaces play a stimulatory role on osteogenic differentiation of MSCs.

引用

页码：1546 / 1554

页数：9

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