Linear dichroism in triplet-singlet difference spectra of photosynthetic pigment-protein complexes, monitored with absorbance-detected magnetic resonance: correction for non-ideal optical effects

被引：0

作者：

Louwe, RJW ^{[1
]}

Vrieze, J ^{[1
]}

van der Vos, R ^{[1
]}

Aartsma, TJ ^{[1
]}

Hoff, AJ ^{[1
]}

机构：

[1] Leiden Univ, Huygens Lab, Dept Biophys, NL-2300 RA Leiden, Netherlands

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 306卷 / 1-2期

关键词：

D O I：

10.1016/S0009-2614(99)00429-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The non-sinusoidal modulation of the photo-elastic modulator used for measurements of the linear dichroism in absorption-detected magnetic resonance (LD-AMDR) causes an experimental error in determining the angles of the triplet spin-sublevel axes relative to the optical dipole transition moment that can be be as large as 20 degrees. We have obtained an analytical correction for this error. The error analysis was applied to LD-ADMR experiments on the carotenoidless mutant R26 of Rhodobacter (Rb.) sphaeroides. The corrected angles of the triplet x- and y-axis with respect to the optical transition dipole moment at 896 nm of R26 are 86 degrees +/- 4 degrees and 22 degrees +/- 3 degrees, respectively. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：25 / 32

页数：8