Four SNPs of insulin-induced gene 1 associated with growth and carcass traits in Qinchuan cattle in China

被引：13
作者：
Liu, Y. [1 ]
Liu, X. L. [1 ]
He, H. [1 ]
Gu, Y. L. [1 ]
机构：
[1] NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling, Shaanxi, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 02期
关键词：
Insig-1; Qinchuan cattle; Association analysis; SNPs; ELEMENT-BINDING PROTEINS; ENDOPLASMIC-RETICULUM; COPII PROTEINS; MESSENGER-RNA; INSIG; HUMANS; POLYMORPHISMS; DEGRADATION; EXPRESSION; SELECTION;
D O I：
10.4238/2012.May.8.3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The insulin-induced gene 1 (Insig-1) is a regulator of lipid metabolism and plays an important role in the sterol-mediated regulation of SREBP, SCAP and HMG-CoA reductase. We used PCR-RFLP and DNA sequencing to detect polymorphisms of the Insig-1 gene in 215 individuals of the Qinchuan cattle breed. Four SNPs [4366(A>G), 4534(T>C), 5001(T>C), and 5235(G>A)] were indentified. The association of the genetic viariation with growth and carcass traits (body length, withers height, hip width, slaughter weight, and carcass weight) was analyzed. The individuals with better performance had the GG genotype at locus A4366G, and CC genotypes at locus T4534C and locus T5001C. These could be used for beef cattle breeding improvement in China. Additionally, linkage disequilibrium analysis reflected that all mutations were in low linkage disequilibrium with each other. We concluded that polymorphisms in the Insig-1 gene are associated with growth and carcass traits and could be used for marker-assisted selection and management in beef cattle breeding programs.
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收藏
页码：1209 / 1216
页数：8
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