Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig's epithelial root sheath

被引：5

作者：

Xu, Jing ^{[1
,2
]}

Kawashima, Nobuyuki ^{[1
]}

Fujiwara, Naoki ^{[3
]}

Harada, Hidemitsu ^{[3
]}

Ota, Masato S.

Suda, Hideaki ^{[1
,2
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Bunkyo Ku, Tokyo 1138549, Japan

[2] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, GCOE Program, Tokyo 1138549, Japan

[3] Iwate Med Univ, Dept Anat, Morioka, Iwate, Japan

来源：

CONGENITAL ANOMALIES | 2012年 / 52卷 / 03期

基金：

日本学术振兴会;

关键词：

Hertwig's epithelial root sheath; tooth root development; vasoactive intestinal peptide; vasoactive intestinal peptide receptor 1; CYCLASE-ACTIVATING POLYPEPTIDE; TOOTH ROOT; PERIODONTAL-LIGAMENT; CELL-PROLIFERATION; DOWN-REGULATION; VIP; INNERVATION; RECEPTORS; NERVES; CULTURE;

D O I：

10.1111/j.1741-4520.2012.00371.x

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Hertwig's epithelial root sheath (HERS), a bilayered epithelial cell sheath located at the cervical loop of the enamel organ in a developing tooth, is at the forefront of root formation. However, little is known about the exact mechanisms that regulate the development of HERS. The neuropeptide vasoactive intestinal peptide (VIP) is involved in the development of various tissues and cells. In this study, we investigated the roles of VIP in HERS development. VIP-immunoreactive nerve fibers were found in the dental pulp and around the root apex of the tooth, while the expression of VIP receptor 1 (VPAC1) was observed in HERS. The expression level of VPAC1 correlated with the development of HERS and was elevated at postnatal days 14 and 21. Using ex vivo cultures of neonatal tooth germs, VIP enhanced the elongation and proliferation of HERS. In vitro, VIP also promoted the proliferation of cells from the HERS-derived cell line, HERS01a cells, and upregulated the mRNA expression of cytokeratin 14 and vimentin (typical molecular markers of HERS) in these cells. These results suggest that VIP may be an essential factor for HERS development.

引用

页码：162 / 167

页数：6

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