Synthesis of of peptide nucleic acids (PNA) with a crosslinking agent to RNA and effective inhibition of dicer

被引：7

作者：

Akisawa, Takuya ^{[1
]}

Yamada, Ken ^{[1
]}

Nagatsugi, Fumi ^{[1
]}

机构：

[1] Tohoku Univ, Inst Multidisciplinaly Res Adv Mat, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2016年 / 26卷 / 24期

基金：

日本学术振兴会;

关键词：

Crosslink; Peptide nucleic acid; miRNA; Dicer; Invasion; ANTISENSE THERAPEUTICS; MICRORNA THERAPEUTICS; NONCODING RNAS; HUMAN-DISEASE; DNA; OLIGONUCLEOTIDES; BIOGENESIS; MIRNA; 2-AMINO-6-VINYLPURINE; ALKYLATION;

D O I：

10.1016/j.bmcl.2016.11.002

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. In this study, we have synthesized PNAs incorporating 2-amino-6-vinylpurine (AVP) for the covalent targeting of single-stranded DNA and RNA, and evaluated their reactivities for these targets. PNA containing AVP at the N-terminal position showed a high reactivity to uracil in RNA and thymine in DNA at the complementary site with AVP. In addition, the crosslinking reactions to pre-miR122 with PNA containing AVP increased the inhibition effect for the Dicer processing of pre-miR122 in vitro. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：5902 / 5906

页数：5

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