β-Subunits of voltage-gated sodium channels in human prostate cancer: quantitative in vitro and in vivo analyses of mRNA expression

被引：44

作者：

Diss, J. K. J. ^{[1
,2
]}

Fraser, S. P. ^{[2
]}

Walker, M. M.

Patel, A. ^{[3
,4
]}

Latchman, D. S. ^{[1
]}

Djamgoz, M. B. A. ^{[2
]}

机构：

[1] UCL, Inst Child Hlth, Med Mol Biol Unit, London WC1N 1EH, England

[2] Univ London Imperial Coll Sci Technol & Med, Neurosci Solut Canc Res Grp, Div Cell & Mol Biol, London, England

[3] St Marys Hosp, Imperial Coll Sch Med, Dept Histopathol, London, England

[4] St Marys Hosp, Imperial Coll Sch Med, Dept Urol, London, England

来源：

PROSTATE CANCER AND PROSTATIC DISEASES | 2008年 / 11卷 / 04期

基金：

英国医学研究理事会;

关键词：

sodium channel; alpha-subunit; beta-subunit; real-time PCR; metastasis; androgen;

D O I：

10.1038/sj.pcan.4501012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We previously identified high levels of Na(v)1.7 voltage-gated sodium channel alpha-subunit ( VGSC alpha) mRNA and protein in human prostate cancer cells and tissues. Here, we investigated auxillary beta-subunit (VGSC beta s) expression. In vitro, the combined expression of all four VGSCbs was significantly (similar to 4.5-fold) higher in strongly compared to weakly metastatic cells. This was mainly due to increased beta 1-expression, which was under androgenic control. In vivo, beta 1-beta 4 mRNAs were detectable and their expression in CaP vs non-CaP tissues generally reflected the in vitro levels in relation to metastatic potential. The possible role(s) of VGSCbs (VGSC alpha-associated and VGSC alpha-independent) in prostate cancer are discussed.

引用

页码：325 / 333

页数：9

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