Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum

被引：8

作者：

Ding, Yu Fei ^{[1
]}

Wang, Chuan Tang ^{[1
]}

Tang, Yue Yi ^{[1
]}

Wang, Xiu Zhen ^{[1
]}

Wu, Qi ^{[1
]}

Hu, Dong Qing ^{[2
]}

Yu, Hong Tao ^{[1
]}

Zhang, Jian Cheng ^{[1
]}

Cui, Feng Gao ^{[1
]}

Song, Guo Sheng ^{[1
]}

Gao, Hua Yuan ^{[3
]}

Yu, Shan Lin ^{[1
]}

机构：

[1] Shandong Peanut Res Inst, Qingdao, Peoples R China

[2] Qingdao Entry Exit Inspect & Quarantine Bur, Qingdao, Peoples R China

[3] Jilin Acad Agr Sci, Inst Econ Plants, Gongzhuling, Peoples R China

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2012年 / 15卷 / 05期

关键词：

Arachis hypogaea; ARF; cyclophilin; differentially expressed genes; genefishing; Ralstonia solanacearum; CERCOSPORIDIUM-PERSONATUM; RESISTANCE; IDENTIFICATION; PCR;

D O I：

10.2225/vol15-issue5-fulltext-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml(-1)) and seeds of the same cultivar treated with water (control), were studied using the Genefishing (TM) technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP- ribosylation factor, respectively, were further studied by real time RTPCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.

引用

页数：11

共 22 条

[1]

Coemans B, 2008, MOL PLANT PATHOL, V9, P25, DOI [10.1111/J.1364-3703.2007.00440.X, 10.1111/j.1364-3703.2007.00440.x]

[2] An elicitor- and pathogen-induced cDNA from potato encodes a stress-responsive cyclophilin [J].