Carvacrol suppresses the expression of inflammatory marker genes in D-galactosamine-hepatotoxic rats

被引：52
作者：
Aristatile, Balakrishnan [1 ,2 ]
Al-Assaf, Abdullah H. [2 ]
Pugalendi, Kodukkur Viswanathan [3 ]
机构：
[1] Bharathiar Univ, Ctr Res & Dev, Coimbatore 641046, Tamil Nadu, India
[2] King Saud Univ, Dept Food Sci & Nutr, Coll Food & Agr Sci, Riyadh 11451, Saudi Arabia
[3] Annamalai Univ, Dept Biochem & Biotechnol, Fac Sci, Annamalainagar 608002, Tamil Nadu, India
来源：
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2013年 / 6卷 / 03期
关键词：
D-galactosamine; Hepatotoxicity; Carvacrol; Anti-inflammatory; NF-KAPPA-B; NITRIC-OXIDE; TNF-ALPHA; LIVER-INJURY; MICE; CYCLOOXYGENASE-2; ACTIVATION; INHIBITORS; PROTEIN; NO;
D O I：
10.1016/S1995-7645(13)60024-3
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Objective: To unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine (D-GaIN)-induced hepatotoxic rats. Methods: The mRNA and protein expression levels of tumor necrosis factor alpha (TNF- alpha), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor kappa-B (NF- kappa B) were assayed by semi-quantitative reverse transcriptase polymerase chain reaction (RTPCR) and western blot analysis. Results: We found that the mRNA and protein expressions of TNF- alpha, IL-6, iNOS, COX-2 and NF- kappa B were significantly up regulated in D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly down-regulated the expressions of these genes showing the mechanism behind the anti-inflammatory activity of carvacrol. Conclusions: All above results reveal that the carvacrol well known anti-inflammatory activities in D galactosamine induced hepatotoxic rats.
引用
收藏
页码：205 / 211
页数：7
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