A novel interference mechanism by a type IIIB CRISPR-Cmr module in Sulfolobus

被引：199

作者：

Deng, Ling ^{[1
]}

Garrett, Roger A. ^{[1
]}

Shah, Shiraz A. ^{[1
]}

Peng, Xu ^{[1
]}

She, Qunxin ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Biol, Archaea Ctr, DK-2200 Copenhagen N, Denmark

来源：

MOLECULAR MICROBIOLOGY | 2013年 / 87卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

MULTIPLE SEQUENCE ALIGNMENT; IMMUNE-SYSTEMS; ANTIVIRAL DEFENSE; FOREIGN DNA; COMPLEX; REPEATS; RNAS; IDENTIFICATION; TRANSCRIPTION; EVOLUTION;

D O I：

10.1111/mmi.12152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies on CRISPR-based adaptive immune systems have revealed extensive structural and functional diversity of the interference complexes which often coexist intracellularly. The archaeon Sulfolobus islandicus REY15A encodes three interference modules, one of type IA and two of type IIIB. Earlier we showed that type IA activity eliminated plasmid vectors carrying matching protospacers with specific CCN PAM sequences. Here we demonstrate that interference-mediated by one type IIIB module Cmr-, and a Csx1 protein, efficiently eliminated plasmid vectors carrying matching protospacers but lacking PAM motifs. Moreover, Cmr--mediated interference was dependent on directional transcription of the protospacer, in contrast to the transcription-independent activities of the type IA and type IIIA DNA interference. We infer that the interference mechanism involves transcription-dependent DNA targeting. A rationale is provided for the intracellular coexistence of the different interference systems in S.islandicus REY15A which cooperate functionally by sharing a single Cas6 protein for crRNA processing and utilize crRNA products from identical CRISPR spacers.

引用

页码：1088 / 1099

页数：12

共 51 条

[1] Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex [J].

Andrecka, Joanna ;

Treutlein, Barbara ;

Izquierdo Arcusa, Maria Angeles ;

Muschielok, Adam ;

Lewis, Robert ;

Cheung, Alan C. M. ;

Cramer, Patrick ;

Michaelis, Jens .

NUCLEIC ACIDS RESEARCH, 2009, 37 (17) :5803-5809

[2] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[3] Identification of Novel Positive-Strand RNA Viruses by Metagenomic Analysis of Archaea-Dominated Yellowstone Hot Springs [J].

Bolduc, Benjamin ;

Shaughnessy, Daniel P. ;

Wolf, Yuri I. ;

Koonin, Eugene V. ;

Roberto, Francisco F. ;

Young, Mark .

JOURNAL OF VIROLOGY, 2012, 86 (10) :5562-5573

[4] Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin [J].

Bolotin, A ;

Ouinquis, B ;

Sorokin, A ;

Ehrlich, SD .

MICROBIOLOGY-SGM, 2005, 151 :2551-2561

[5] Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system [J].

Bondy-Denomy, Joe ;

Pawluk, April ;

Maxwell, Karen L. ;

Davidson, Alan R. .

NATURE, 2013, 493 (7432) :429-U181

[6] Small CRISPR RNAs guide antiviral defense in prokaryotes [J].

Brouns, Stan J. J. ;

Jore, Matthijs M. ;

Lundgren, Magnus ;

Westra, Edze R. ;

Slijkhuis, Rik J. H. ;

Snijders, Ambrosius P. L. ;

Dickman, Mark J. ;

Makarova, Kira S. ;

Koonin, Eugene V. ;

van der Oost, John .

SCIENCE, 2008, 321 (5891) :960-964

[7] COMPARISON OF PLASMID DNA TOPOLOGY AMONG MESOPHILIC AND THERMOPHILIC EUBACTERIA AND ARCHAEBACTERIA [J].

CHARBONNIER, F ;

FORTERRE, P .

JOURNAL OF BACTERIOLOGY, 1994, 176 (05) :1251-1259

[8] Characterization of the Sulfolobus host-SSV2 virus interaction [J].

Contursi, Patrizia ;

Jensen, Susanne ;

Aucelli, Tiziana ;

Rossi, Mose ;

Bartolucci, Simonetta ;

She, Qunxin .

EXTREMOPHILES, 2006, 10 (06) :615-627

[9] Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus [J].

Deng, Ling ;

Kenchappa, Chandra S. ;

Peng, Xu ;

She, Qunxin ;

Garrett, Roger A. .

NUCLEIC ACIDS RESEARCH, 2012, 40 (06) :2470-2480

[10] Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus [J].

Deng, Ling ;

Zhu, Haojun ;

Chen, Zhengjun ;

Liang, Yun Xiang ;

She, Qunxin .

EXTREMOPHILES, 2009, 13 (04) :735-746

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