Coexpression of protein disulfide isomerase (PDI) enhances production of kringle fragment of human apoliprotein(a) in recombinant Saccharomyces cerevisiae

被引：0

作者：

Cha, KH

Kim, MD

Lee, TH

Lim, HK

Jung, KH

Seo, JH ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea

[2] Seoul Natl Univ, Ctr Agr Biomat, Seoul 151921, South Korea

[3] Mogan Biotechnol Res Inst, Yongin 449913, South Korea

[4] Chungju Natl Univ, Dept Food & Biotechnol, Chungju 380702, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 16卷 / 02期

关键词：

apolipoprotein(a); kringle; Saccharomyces cerevisidae; protein disulfide isomerase;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In an attempt to increase production of LK8, an 86-amino-acid kringle fragment of human apolipoprotein(a) with three disulfide linkages, protein disulfide isomerase (PDI) was coexpressed in recombinant Saccharomyces cerevisiae harboring the LK8 gene in the chromosome. Whereas overexpression of the LK8 gene without coexpressing PDI was detrimental to both host cell growth and LK8 production, coexpression of PDI increased the LK8 production level by 2.5-fold in batch cultivation and 5.0-fold in fed-batch cultivation compared with the control strain carrying only the genomic PDI gene.

引用

页码：308 / 311

页数：4

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[1] Angiogenesis in cancer and other diseases [J].