Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit

被引：3
作者：
Chicherin, I. V. [1 ]
Zinina, V. V. [1 ]
Levitskiy, S. A. [1 ]
Serebryakova, M. V. [2 ]
Kamenski, P. A. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia
[2] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow 119991, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2019年 / 84卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
mitochondria; translation; ribosomes; initiation factor; Aim23p; MESSENGER-RNA; TRANSLATION; IF3; SEQUENCE; GENOME;
D O I：
10.1134/S000629791901005X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein synthesis in mitochondria is generally organized in a bacterial-like manner but, at the same time, possesses several unique traits. Translation initiation in mitochondria is regulated by two protein factors, mtIF2 and mtIF3. Previously we demonstrated that Saccharomyces cerevisiae Aim23 protein is an ortholog of IF3 in budding yeast. However, the data on the interactions between Aim23p and other proteins are limited. Here, we demonstrated that Aim23p interacts with the yeast mitochondrial ribosomal small subunit both in vivo and in vitro using co-immunoprecipitation and density gradient sedimentation.
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收藏
页码：40 / 46
页数：7
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