Synergistic effect of HA and BMP-2 mimicking peptide on the bioactivity of HA/PMMA bone cement

被引：30

作者：

Liu, Zhenzhen ^{[1
]}

Tang, Yong ^{[2
]}

Kang, Ting ^{[1
]}

Rao, Minyu ^{[1
]}

Li, Kejia ^{[1
]}

Wang, Qin ^{[1
]}

Quan, Changyun ^{[1
]}

Zhang, Chao ^{[1
]}

Jiang, Qing ^{[1
]}

Shen, Huiyong

机构：

[1] Sun Yat Sen Univ, Sch Engn, Dept Biomed Engn, Guangzhou 510006, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Dept Orthoped, Sun Yat Sen Mem Hosp, Guangzhou 510120, Guangdong, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 131卷

基金：

中国国家自然科学基金;

关键词：

Poly(methyl methacrylate); Nano-hydroxyapatite; BMP-2 mimicking peptide; Synergistic action; Bioactivity; MESENCHYMAL STEM-CELLS; IN-VITRO; HYDROXYAPATITE; COMPOSITES; DELIVERY; NANOPARTICLES; FORMULATIONS; METHACRYLATE; OSTEOBLASTS; NANOTUBES;

D O I：

10.1016/j.colsurfb.2015.04.032

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

HA and bone morphogenetic protein-2 (BMP-2) possess superior osteoinductive and osteoconductive activities in the repair of bone defects. In this study, a BMP-2 mimicking oligopeptide (serine-serine-valine-proline-threonine, SSVPT) was introduced to the surface of nano-sized HA as the reinforcement phase of a poly(methyl methacrylate) (PMMA) cement, and the synergistic action of HA and oligopeptide on the bioactivity of HA/PMMA bone cement was investigated. Incorporated with 3-trimethoxysilyl propyl methacrylate (MPS)-modified HA, the compressive strength, flexural strength, and flexural modulus of HA/PMMA complied with the international standardization organization 5833 standard. Adherence measurement demonstrated that the introduction of HA and SSVPT could promote the adhesion and proliferation of human fetal osteoblast (hFOB) on the surface of HA/PMMA cement. The increased alkaline phosphatase (ALP) activity indicated that HA/PMMA could promote osteogenic differentiation of hFOB. All of the results illuminated that the HA/PMMA cement could be used as a bioactive material for regeneration and reconstruction of load-bearing bone. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：39 / 46

页数：8

共 39 条

[1] New bioactive glass-ceramic: Synthesis and application in PMMA bone cement composites [J].

Abd Samad, Hamizah ;

Jaafar, Mariatti ;

Othman, Radzali ;

Kawashita, Masakazu ;

Abdul Razak, Noor Hayati .

BIO-MEDICAL MATERIALS AND ENGINEERING, 2011, 21 (04) :247-258

[2] Study of gamma radiation effect on PMMA/HA composites prepared by solution [J].

Albano, C ;

Karam, A ;

González, G ;

Domínguez, N ;

Sánchez, Y .

MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 2006, 448 :243-249

[3] Preparation, modification, and characterization of acrylic cements [J].

Basgorenay, B ;

Ulubayram, K ;

Serbetci, K ;

Onurhan, E ;

Hasirci, N .

JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 99 (06) :3631-3637

[4] Gene expression of bone-associated cytokines in MG63 osteoblast-like cells incubated with acrylic bone cement extracts in minimum essential medium [J].

Cenni, E ;

Granchi, D ;

Ciapetti, G ;

Savarino, L ;

Corradini, A ;

Vancini, M ;

Giunti, A .

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2002, 13 (12) :1283-1294

[5] Amphiphilic cationic lipopeptides with RGD sequences as gene vectors [J].

Chen, Jing-Xiao ;

Wang, Hui-Yuan ;

Quan, Chang-Yun ;

Xu, Xiao-Ding ;

Zhang, Xian-Zheng ;

Zhuo, Ren-Xi .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2010, 8 (14) :3142-3148

[6] Increasing hydroxyapatite incorporation into poly(methylmethacrylate) cement increases osteoblast adhesion and response [J].

Dalby, MJ ;

Di Silvio, L ;

Harper, EJ ;

Bonfield, W .

BIOMATERIALS, 2002, 23 (02) :569-576

[7] Initial interaction of osteoblasts with the surface of a hydroxyapatite-poly(methylmethacrylate) cement [J].

Dalby, MJ ;

Di Silvio, L ;

Harper, EJ ;

Bonfield, W .

BIOMATERIALS, 2001, 22 (13) :1739-1747

[8] Evaluation of the in vitro biocompatibility of PMMA/high-load HA/carbon nanostructures bone cement formulations [J].

Goncalves, Gil ;

Portoles, Maria-Teresa ;

Ramirez-Santillan, Cecilia ;

Vallet-Regi, Maria ;

Serro, Ana Paula ;

Gracio, Jose ;

Marques, Paula A. A. P. .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2013, 24 (12) :2787-2796

[9] Graphene oxide versus functionalized carbon nanotubes as a reinforcing agent in a PMMA/HA bone cement [J].

Goncalves, Gil ;

Cruz, Sandra M. A. ;

Ramalho, A. ;

Gracio, Jose ;

Marques, Paula A. A. P. .

NANOSCALE, 2012, 4 (09) :2937-2945

[10] Bioactive bone cements containing nano-sized titania particles for use as bone substitutes [J].

Goto, K ;

Tamura, J ;

Shinzato, S ;

Fujibayashi, S ;

Hashimoto, M ;

Kawashita, M ;

Kokubo, T ;

Nakamura, T .

BIOMATERIALS, 2005, 26 (33) :6496-6505

← 1 2 3 4 →