Quantitative trait loci analysis of seed-specialized metabolites reveals seed-specific flavonols and differential regulation of glycoalkaloid content in tomato

被引:31
作者
Alseekh, Saleh [1 ,2 ]
Ofner, Itai [3 ]
Liu, Zhongyuan [4 ]
Osorio, Sonia [5 ]
Vallarino, Jose [1 ]
Last, Robert L. [6 ,7 ]
Zamir, Dani [3 ]
Tohge, Takayuki [1 ]
Fernie, Alisdair R. [1 ,2 ]
机构
[1] Max Planck Inst Mol Plant Physiol, Muhlenberg 1, D-14476 Potsdam, Germany
[2] Ctr Plant Syst Biol & Biotechnol, Plovdiv 4000, Bulgaria
[3] Hebrew Univ Jerusalem, Fac Agr, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel
[4] Univ Florida, Plant Innovat Ctr, Hort Sci, Gainesville, FL 32611 USA
[5] Univ Malaga, Dept Mol Biol & Biochem, Inst Hortofruiticultura Subtrop & Mediterranea Ma, IHSM,UMA,CSIC, Campus Teatinos, Malaga 29071, Spain
[6] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
[7] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
基金
美国国家科学基金会;
关键词
flavonol; tomato; steroidal glycoalkaloids; introgression lines; seeds; specialized metabolites; LYCOPERSICON-PENNELLII; ALPHA-TOMATINE; FRUIT; PLANT; BIOSYNTHESIS; IDENTIFICATION; ARABIDOPSIS; ANTIOXIDANT; DIVERSITY; GENOME;
D O I
10.1111/tpj.14879
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Given the potential health benefits (and adverse effects), of polyphenolic and steroidal glycoalkaloids in the diet there is a growing interest in fully elucidating the genetic control of their levels in foodstuffs. Here we carried out profiling of the specialized metabolites in the seeds of theSolanum pennelliiintrogression lines identifying 338 putative metabolite quantitative trait loci (mQTL) for flavonoids, steroidal glycoalkaloids and further specialized metabolites. Two putative mQTL for flavonols and one for steroidal glycoalkaloids were cross-validated by evaluation of the metabolite content of recombinants harboring smaller introgression in the corresponding QTL interval or by analysis of lines from an independently derived backcross inbred line population. The steroidal glycoalkaloid mQTL was localized to a chromosomal region spanning 14 genes, including a previously defined steroidal glycoalkaloid gene cluster. The flavonoid mQTL was further validated via the use of transient and stable overexpression of theSolyc12g098600andSolyc12g096870genes, which encode seed-specific uridine 5 '-diphosphate-glycosyltransferases. The results are discussed in the context of our understanding of the accumulation of polyphenols and steroidal glycoalkaloids, and how this knowledge may be incorporated into breeding strategies aimed at improving nutritional aspects of plants as well as in fortifying them against abiotic stress.
引用
收藏
页码:2007 / 2024
页数:18
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