Expression, SNV identification, linkage disequilibrium, and combined genotype association analysis of the muscle-specific gene CSRP3 in Chinese cattle

被引：24
作者：
He, Hua [1 ]
Zhang, Hui-lin [1 ]
Li, Zhi-xiong [1 ]
Liu, Yu [1 ]
Liu, Xiao-lin [1 ]
机构：
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
来源：
GENE | 2014年 / 535卷 / 01期
关键词：
Cattle; CSRP3; gene; Single nucleotide variants; Combined genotypes; Association analysis; Gene expression; CYSTEINE-RICH PROTEIN; LIM PROTEIN; GROWTH TRAITS; DILATED CARDIOMYOPATHY; DISRUPTION; MYOGENESIS;
D O I：
10.1016/j.gene.2013.11.014
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The cysteine and glycine-rich protein 3 (CSRP3) plays an important role in the myofiber differentiation. Here, we identified five SNVs in all exon and intron regions of the CSRP3 gene using DNA sequencing, PCR-RFLP and forced-PCR-RFLP methods in 554 cattle. Four of the five SNVs were significantly associated with growth performance and carcass traits of the cattle. In addition, we evaluated haplotype frequency and linkage disequilibrium coefficient of five sequence variants. The result of haplotype analysis demonstrated 28 haplotypes present in Qinchuan and two haplotypes in Chinese Holstein. Only haplotypes I and 8 were being shared by two populations, haplotype 14 had the highest haplotype frequency in Qinchuan (17.4%) and haplotype 8 had the highest haplotype frequency in Chinese Holstein (94.4%). Statistical analyses of combined genotypes indicated that some combined genotypes were significantly or highly significantly associated with growth and carcass traits in the Qinchuan cattle population. qPCR analyses also showed that bovine CSRP3 gene was exclusively expressed in longissimus dorsi muscle and heart tissues. The data support the high potential of the CSRP3 as a marker gene for the improvement of growth performance and carcass traits in selection programs. (C) 2013 Elsevier B.V. All rights reserved.
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页码：17 / 23
页数：7
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