Detecting Aspergillus parasiticus in cereals by an enzyme-linked immunosorbent assay

被引:14
|
作者
Tsai, GJ [1 ]
Yu, SC [1 ]
机构
[1] Natl Taiwan Ocean Univ, Dept Food Sci, Keelung 202, Taiwan
关键词
enzyme-linked immunosorbent assay; Aspergillus parasiticus; aflatoxigenic molds; cereals;
D O I
10.1016/S0168-1605(99)00084-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
An enzyme-linked immunosorbent assay (ELISA) was used for the specific detection of Aspergillus parasiticus molds in artificially contaminated corn, rice, wheat and peanut, and also to detect naturally occurring aflatoxigenic molds in the same form cereals. After inoculation of Aspergillus parasiticus in the cereals, the growth of A. parasiticus was monitored by both plate count and ELISA, and the aflatoxin content was measured. Water activity (a(w)) affected the fungal growth, aflatoxin production and degradation. The higher a(w) level (a(w) = 0.98 vs, a(w) = 0.92) resulted in higher fungal growth rates and fungal masses in corn, rice and wheat, and the plate count and ELISA measurements were better correlated, with correlation coefficients of 0.94, 0.93, 0.96 and 0.86, respectively for corn, rice, wheat, and peanuts. Aflatoxin was also both produced sooner and degraded more rapidly at a(w) = 0.98. Although standard plate counting techniques detected A. parasiticus/A. flavus in 5 out of the 40 cereal samples bought from retail stores, ELISA did not give a positive result in any of them. After moisturization and incubation of these commercial samples at 28 degrees C for 29 days, the incident rates of aflatoxigenic molds increased to 65% and 52% by plate count and ELISA, respectively. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:181 / 189
页数:9
相关论文
共 50 条
  • [1] An enzyme-linked immunosorbent assay for the detection of Aspergillus parasiticus and Aspergillus flavus
    Tsai, GJ
    Yu, SC
    JOURNAL OF FOOD PROTECTION, 1997, 60 (08) : 978 - 984
  • [2] Monoclonal-based enzyme-linked immunosorbent assay for the detection of zearalenone in cereals
    Thongrussamee, T.
    Kuzmina, N. S.
    Shim, W. -B.
    Jiratpong, T.
    Eremin, S. A.
    Intrasook, J.
    Chung, D. -H.
    FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2008, 25 (08): : 997 - 1006
  • [3] Validation of an Enzyme-Linked Immunosorbent Assay for Detecting Sulfonamides in Feed Resources
    Jimenez, Vanesa
    Adrian, Javier
    Guiteras, Jacinto
    Marco, Maria-Pilar
    Companyo, Ramon
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (13) : 7526 - 7531
  • [4] Enzyme-linked immunosorbent assay for the determination of T-2 toxin in cereals and feedstuff
    Junping Wang
    Suxin Duan
    Yan Zhang
    Shuo Wang
    Microchimica Acta, 2010, 169 : 137 - 144
  • [5] QuEChERS extraction followed by enzyme-linked immunosorbent assay for determination of deoxynivalenol and zearalenone in cereals
    He, Qiurong
    Peng, Hongwei
    Yang, Jinyi
    Xu, Zhenlin
    Fan, Congcong
    Sun, Yuanming
    FOOD AND AGRICULTURAL IMMUNOLOGY, 2017, 28 (06) : 1477 - 1495
  • [6] Enzyme-linked immunosorbent assay for the determination of T-2 toxin in cereals and feedstuff
    Wang, Junping
    Duan, Suxin
    Zhang, Yan
    Wang, Shuo
    MICROCHIMICA ACTA, 2010, 169 (1-2) : 137 - 144
  • [7] A competitive enzyme-linked immunosorbent assay for diminazene
    Karanja, WM
    Mdachi, RE
    Murilla, GA
    ACTA TROPICA, 2002, 84 (02) : 75 - 81
  • [8] Enzyme-linked immunosorbent assay for detection of chitooligosaccharides
    Kim, SY
    Shon, DH
    Lee, KH
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2000, 64 (04) : 696 - 701
  • [9] The research on enzyme-linked immunosorbent assay for fenvalerate
    Lu, Xi-Qin
    Zhang, Bang-Hong
    Shi, Hai-Yan
    Wang, Ming-Hua
    FOOD AND AGRICULTURAL IMMUNOLOGY, 2009, 20 (02) : 147 - 154
  • [10] Enzyme-Linked Immunosorbent Assay of Chlorampenicol in Foodstuff
    Fedorova, M. D.
    Andreeva, I. P.
    Vilegzhanina, E. S.
    Komarov, A. A.
    Rubtsova, M. Yu.
    Samsonova, J. V.
    Egorov, A. M.
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2010, 46 (08) : 795 - 801