Effect of solvent on ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry of peptides

被引：5

作者：

Hazama, Hisanao ^{[1
]}

Furukawa, Satoshi ^{[1
]}

Awazu, Kunio ^{[1
,2
,3
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[3] Ctr Adv Med Engn & Informat, Suita, Osaka 5650871, Japan

来源：

CHEMICAL PHYSICS | 2013年 / 419卷

关键词：

Matrix-assisted laser desorption/ionization; Mass spectrometry; Peptide; Solvent; Residual water; QUANTITATIVE MODEL; DESORPTION; MALDI; IONS;

D O I：

10.1016/j.chemphys.2013.03.004

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Although various theoretical models of matrix-assisted laser desorption/ionization (MALDI) have been proposed, no model can explain all of the experimentally observed phenomena. It has been reported that water content in the solvent used in the sample preparation has influence on the ion yield of MALDI. However, the previously proposed models do not take account of the solvent. Therefore, we have investigated the influence of the solvent on MALDI mass spectrometry of peptides. Among the solvents of water, methanol, ethanol, tetrahydrofuran, and acetonitrile, the signal peak area of the peptide ion was the largest when the water solvent was used. It is suggested that the residual water solvent contained in the dried matrix crystal has influence on the ionization processes of MALDI. It is inferred that inclusion of the influence of the residual solvent into the theoretical model of MALDI could contribute to the improvement of the precision of the model. (C) 2013 Elsevier B. V. All rights reserved.

引用

页码：196 / 199

页数：4

共 11 条

[1] Matrix-assisted laser desorption/ionization (MALDI) mechanism revisited [J].