Detection of Streptococcus equi subspecies equi using a triplex qPCR assay

被引：45
作者：
Webb, Katy [1 ]
Barker, Colin [1 ]
Harrison, Tihana [1 ]
Heather, Zoe [1 ]
Steward, Karen F. [1 ]
Robinson, Carl [1 ]
Newton, J. Richard [1 ]
Waller, Andrew S. [1 ]
机构：
[1] Anim Hlth Trust, Ctr Prevent Med, Newmarket CB8 7UU, Suffolk, England
来源：
VETERINARY JOURNAL | 2013年 / 195卷 / 03期
基金：
英国惠康基金;
关键词：
Equine; Strangles; Streptococcus equi; Triplex quantitative PCR; STRANGLES OUTBREAKS; HORSES; AMPLIFICATION; PCR;
D O I：
10.1016/j.tvjl.2012.07.007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：300 / 304
页数：5
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