Disruption of multidrug resistance protein gene of Rhodobacter capsulatus results in improved photoheterotrophic hydrogen production

被引：10
作者：
Ma, Chao [1 ]
Yang, Honghui [1 ]
Zhang, Yang [1 ]
Guo, Liejin [1 ]
机构：
[1] Xi An Jiao Tong Univ, State Key Lab Multiphase Flow Power Engn, Xian 710049, Peoples R China
来源：
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2013年 / 38卷 / 29期
基金：
中国国家自然科学基金;
关键词：
Biohydrogen; Photoheterotrophic hydrogen production; Reduced pigment; Multidrug resistance protein gene; Rhodobacter capsulatus; PHOTOSYNTHETIC BACTERIUM MUTANT; GRAM-NEGATIVE BACTERIA; REDUCED PIGMENT; EFFLUX PUMPS; BIOHYDROGEN PRODUCTION; ANTIBIOTIC-RESISTANCE; SPHAEROIDES; BIOSYNTHESIS; STRAINS; SYSTEM;
D O I：
10.1016/j.ijhydene.2013.03.116
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Rhodobacter capsulatus (R. capsulatus), which is a typical purple nonsulfur photosynthetic bacterium, is able to produce hydrogen under photosynthetic condition. A mutant of R. capsulatus named MC122 was obtained by Tn5 transposon mutagenesis. The transposon mutant had improved photoheterotrophic hydrogen production performance using acetic acid as substrate and its mutation site was located by sequencing the rescued plasmid containing the transposon insertion from the genome of the mutant. It was found for the first time that disruption of the multidrug resistance protein (mdtB) gene resulted in improved hydrogen production. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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页码：13031 / 13037
页数：7
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