Carbodiimide crosslinked collagen from porcine dermal matrix for high-strength tissue engineering scaffold

被引：61

作者：

Li, Jianhua ^{[1
]}

Ren, Na ^{[1
]}

Qiu, Jichuan ^{[1
]}

Jiang, Huaidong ^{[1
]}

Zhao, Hongshi ^{[2
]}

Wang, Guancong ^{[3
]}

Boughton, Robert I. ^{[4
]}

Wang, Yingjun ^{[5
]}

Liu, Hong ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Crystal Mat, Ctr Bio & Micro Nano Funct Mat, Jinan 250100, Peoples R China

[2] Univ Wurzburg, Inst Pharm & Food Chem, DE-97070 Wurzburg, Germany

[3] Shandong Energy Grp Co Ltd, Ctr Res & Technol Dev, Jinan 250014, Peoples R China

[4] Bowling Green State Univ, Dept Phys & Astron, Bowling Green, OH 43403 USA

[5] S China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510640, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 61卷

基金：

中国国家自然科学基金;

关键词：

Porcine dermal collagen; Carbodiimide; Crosslinking; Mechanical properties; Degradation; Cytocompatibility; LINKING; KNEE;

D O I：

10.1016/j.ijbiomac.2013.06.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Naturally-derived collagens for tissue engineering are limited by low mechanical strength and rapid degradation. In this study, carbodiimide is used to chemically modify the collagen derived from porcine acellular dermal matrix (PADM). The results show that the strength and resistance of PADM to enzymatic digestion can be adjusted by the reconnection of free amino and carboxyl groups of the collagen fibers. The cytocompatibility of the crosslinked PADM was evaluated by cell adhesion and proliferation assays. The cell culture studies on crosslinked and uncrosslinked PADM showed that the modification does not affect the scaffold's biocompatibility. These results demonstrate that the PADM collagen materials can be strengthened through a low-cost, non-toxic crosslinking method for potential use in biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：69 / 74

页数：6

共 32 条

[11] Nanotechnological strategies for engineering complex tissues [J].

Dvir, Tal ;

Timko, Brian P. ;

Kohane, Daniel S. ;

Langer, Robert .

NATURE NANOTECHNOLOGY, 2011, 6 (01) :13-22

[12]

Grafahrend D., 2010, Nat. Mater, V10, P67

[13] Skin tissue engineering - In vivo and in vitro applications [J].

Groeber, Florian ;

Holeiter, Monika ;

Hampel, Martina ;

Hinderer, Svenja ;

Schenke-Layland, Katja .

ADVANCED DRUG DELIVERY REVIEWS, 2011, 63 (4-5) :352-366

[14]

Haugh MG, 2011, TISSUE ENG PT A, V17, P1201, DOI [10.1089/ten.tea.2010.0590, 10.1089/ten.TEA.2010.0590]

[15] Comparison of different decellularization procedures of porcine heart valves [J].

Kasimir, MT ;

Rieder, E ;

Seebacher, G ;

Silberhumer, G ;

Wolner, E ;

Weigel, G ;

Simon, P .

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 2003, 26 (05) :421-427

[16]

Liu XH, 2011, NAT MATER, V10, P398, DOI [10.1038/NMAT2999, 10.1038/nmat2999]

[17]

MOORE S, 1954, J BIOL CHEM, V211, P893

[18] Crosslinking of collagen gels by transglutaminase [J].

Orban, JM ;

Wilson, LB ;

Kofroth, JA ;

El-Kurdi, MS ;

Maul, TM ;

Vorp, DA .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2004, 68A (04) :756-762

[19] Regeneration and orthotopic transplantation of a bioartificial lung [J].

Ott, Harald C. ;

Clippinger, Ben ;

Conrad, Claudius ;

Schuetz, Christian ;

Pomerantseva, Irina ;

Ikonomou, Laertis ;

Kotton, Darrell ;

Vacanti, Joseph P. .

NATURE MEDICINE, 2010, 16 (08) :927-U131

[20] EDC cross-linking improves skin substitute strength and stability [J].

Powell, Heather M. ;

Boyce, Steven T. .

BIOMATERIALS, 2006, 27 (34) :5821-5827

← 1 2 3 4 →