Studies on the immobilisation of acetylcholine esterase enzyme for biosensor applications

被引：11

作者：

Rekha, K. ^{[1
]}

Murthy, B. Narasimha ^{[2
]}

机构：

[1] CMR Inst Technol, Dept Biotechnol, Bangalore, Karnataka, India

[2] CMR Inst Technol, Dept Chem, Bangalore, Karnataka, India

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2008年 / 19卷 / 04期

关键词：

biosensor; acetylcholine esterase (AChE) enzyme; immobilisation co-cross-linking method;

D O I：

10.1080/09540100802380846

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Conventional methods of organophosphate (OP) pesticide analysis have several disadvantages; some of them can be overcome by the use of biosensors. This paper deals with studies on different immobilisation methods for biosensor applications. Acetylcholine esterase (AChE) was immobilised using two different membranes in order to construct a sensitive biosensor for the detection of OP pesticides. Amongst tested methods of immobilisation a co-cross-linking method of immobilisation using cellophane membrane gave the best results for the detection of the OP pesticide, paraoxon, in the range of 0.4-2 ppm with an analysis time of 15 minutes.

引用

页码：273 / 281

页数：9

共 14 条

[1] CHEMILUMINESCENCE-BASED INHIBITION-KINETICS OF ALKALINE-PHOSPHATASE IN THE DEVELOPMENT OF A PESTICIDE BIOSENSOR [J].

AYYAGARI, MS ;

KAMTEKAR, S ;

PANDE, R ;

MARX, KA ;

KUMAR, J ;

TRIPATHY, SK ;

AKKARA, J ;

KAPLAN, DL .

BIOTECHNOLOGY PROGRESS, 1995, 11 (06) :699-703

[2] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[3] Increased paraoxon detection with solvents using acetylcholinesterase inactivation measured with a choline oxidase biosensor [J].

Fennouh, S ;

Casimiri, V ;

Burstein, C .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (02) :97-104

[4] A dual enzyme amperometric biosensor for monitoring organophosphorous pesticides [J].

Gouda, MD ;

Thakur, MS ;

Karanth, NG .

BIOTECHNOLOGY TECHNIQUES, 1997, 11 (09) :653-655

[5] Thermal inactivation of glucose oxidase - Mechanism and stabilization using additives [J].

Gouda, MD ;

Singh, SA ;

Rao, AGA ;

Thakur, MS ;

Karanth, NG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (27) :24324-24333

[6] Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents [J].

Gouda, MD ;

Kumar, MA ;

Thakur, MS ;

Karanth, NG .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (6-7) :503-507

[7]

Hartley Ian C., 1994, Analytical Proceedings, V31, P333, DOI 10.1039/ai9943100333

[8]

HORNBY WE, 1976, METHOD ENZYMOL, V44, P3

[9] POTENTIOMETRIC BUTYRYLCHOLINE SENSOR FOR ORGANOPHOSPHATE PESTICIDES [J].

IMATO, T ;

ISHIBASHI, N .

BIOSENSORS & BIOELECTRONICS, 1995, 10 (05) :435-441

[10] ORGANOPHOSPHATE PESTICIDE EXPOSURE IN A GROUP OF WASHINGTON-STATE ORCHARD APPLICATORS [J].

KARR, C ;

DEMERS, P ;

COSTA, LG ;

DANIELL, WE ;

BARNHART, S ;

MILLER, M ;

GALLAGHER, G ;

HORSTMAN, SW ;

EATON, D ;

ROSENSTOCK, L .

ENVIRONMENTAL RESEARCH, 1992, 59 (01) :229-237

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