Transport evaluation of salicylic acid and structurally related compounds across Caco-2 cell monolayers and artificial PAMPA membranes

被引:13
|
作者
Koljonen, Maija [1 ]
Rousu, Katja [2 ]
Cierny, Jakub
Kaukonen, Ann Marie [3 ]
Hirvonen, Jouni
机构
[1] Univ Helsinki, Fac Pharm, Div Pharmaceut Technol, FIN-00014 Helsinki, Finland
[2] Orion Corp Orion Pharma, Res & Dev, Espoo, Finland
[3] Univ Helsinki, Drug Discovery & Dev Technol Ctr DDTC, FIN-00014 Helsinki, Finland
关键词
Caco-2; PAMPA; Transport mechanisms; Anionic compounds; pH-dependency;
D O I
10.1016/j.ejpb.2008.05.017
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The purpose of this study was to evaluate passive vs. proton-dependent active transport mechanisms of salicylic acid (SA) and four structurally related anions. Transport was studied across Caco-2 cell monolayers and artificial lipid membranes (PAMPA) under pH-gradient and iso-pH conditions. Kinetic permeability parameters were provided by bidirectional Caco-2 experiments and concentration-dependency measurements. The transport route and putative transporters involved in SA transport were studied using EDTA and several inhibitors. SA and lipophilic 5-chlorosalicylic acid and 2-hydroxy-1-naphthoic acid reached saturation with increasing compound concentration indicating active transport. Permeation of 5-hydroxysalicylic acid and 5-hydroxyisophthalic acid was not saturated indicating passive transport. PAMPA with pure passive diffusion underestimated the transport of SA compared to Caco-2. Opening up the paracellular tight junctions by EDTA did not increase the transport of SA under the pH-gradient conditions confirming the hypothesis of pure transcellular transport of SA. Active transport of SA remained concentration-dependent even without the pH-gradient, and was reduced by the known MCT1 and OATP-B inhibitors and structurally related anions. Overall, several permeability test protocols are needed to obtain a more complete picture of transport properties of salicylic acid and structurally related compounds. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:531 / 538
页数:8
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