Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications

被引：14

作者：

Furuno, Kotoko ^{[1
]}

Wang, Jing ^{[1
]}

Suzuki, Keiichiro ^{[2
,3
]}

Nakahata, Masaki ^{[1
]}

Sakai, Shinji ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn Sci, Dept Mat Engn Sci, Toyonaka, Osaka 5608531, Japan

[2] Osaka Univ, Dept Mat Engn Sci, Grad Sch Engn Sci, Inst Adv Cocreat Studies, Toyonaka, Osaka 5608531, Japan

[3] Osaka Univ, Grad Sch Frontier Biosci, Toyonaka, Osaka 5608531, Japan

来源：

ACS OMEGA | 2020年 / 5卷 / 33期

关键词：

NANOFIBROUS SCAFFOLD; FIBROUS MEMBRANES; STRATEGIES;

D O I：

10.1021/acsomega.0c03164

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells.

引用

页码：21254 / 21259

页数：6

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