Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications

被引：14
|
作者：
Furuno, Kotoko [1 ]
Wang, Jing [1 ]
Suzuki, Keiichiro [2 ,3 ]
Nakahata, Masaki [1 ]
Sakai, Shinji [1 ]
机构：
[1] Osaka Univ, Grad Sch Engn Sci, Dept Mat Engn Sci, Toyonaka, Osaka 5608531, Japan
[2] Osaka Univ, Dept Mat Engn Sci, Grad Sch Engn Sci, Inst Adv Cocreat Studies, Toyonaka, Osaka 5608531, Japan
[3] Osaka Univ, Grad Sch Frontier Biosci, Toyonaka, Osaka 5608531, Japan
来源：
ACS OMEGA | 2020年 / 5卷 / 33期
关键词：
NANOFIBROUS SCAFFOLD; FIBROUS MEMBRANES; STRATEGIES;
D O I：
10.1021/acsomega.0c03164
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells.
引用
收藏
页码：21254 / 21259
页数：6
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