De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris

被引:19
|
作者
Niu, Shan-Ce [1 ,2 ]
Xu, Qing [3 ,4 ]
Zhang, Guo-Qiang [3 ,4 ]
Zhang, Yong-Qiang [3 ,4 ]
Tsai, Wen-Chieh [5 ,6 ,7 ]
Hsu, Jui-Ling [3 ,4 ,6 ]
Liang, Chieh-Kai [5 ]
Luo, Yi-Bo [1 ]
Liu, Zhong-Jian [3 ,4 ,8 ,9 ,10 ]
机构
[1] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen 518114, Peoples R China
[4] Orchid Conservat & Res Ctr Shenzhen, Shenzhen 518114, Peoples R China
[5] Natl Cheng Kung Univ, Inst Trop Plant Sci, Tainan 701, Taiwan
[6] Natl Cheng Kung Univ, Orchid Res & Dev Ctr, Tainan 701, Taiwan
[7] Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
[8] Tsinghua Univ, Grad Sch Shenzhen, Ctr Biotechnol & BioMed, Shenzhen 518055, Peoples R China
[9] South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510640, Guangdong, Peoples R China
[10] Fujian Agr & Forestry Univ, Coll Arts, Coll Landscape Architecture, Fuzhou 350002, Peoples R China
关键词
EXPRESSION ANALYSIS; FLOWER BUDS; FAMILY; GENERATION; EPIPHYTISM; PROTEINS; ESTS;
D O I
10.1038/sdata.2016.83
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search.
引用
收藏
页数:11
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