De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris

被引:19
|
作者
Niu, Shan-Ce [1 ,2 ]
Xu, Qing [3 ,4 ]
Zhang, Guo-Qiang [3 ,4 ]
Zhang, Yong-Qiang [3 ,4 ]
Tsai, Wen-Chieh [5 ,6 ,7 ]
Hsu, Jui-Ling [3 ,4 ,6 ]
Liang, Chieh-Kai [5 ]
Luo, Yi-Bo [1 ]
Liu, Zhong-Jian [3 ,4 ,8 ,9 ,10 ]
机构
[1] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen 518114, Peoples R China
[4] Orchid Conservat & Res Ctr Shenzhen, Shenzhen 518114, Peoples R China
[5] Natl Cheng Kung Univ, Inst Trop Plant Sci, Tainan 701, Taiwan
[6] Natl Cheng Kung Univ, Orchid Res & Dev Ctr, Tainan 701, Taiwan
[7] Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
[8] Tsinghua Univ, Grad Sch Shenzhen, Ctr Biotechnol & BioMed, Shenzhen 518055, Peoples R China
[9] South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510640, Guangdong, Peoples R China
[10] Fujian Agr & Forestry Univ, Coll Arts, Coll Landscape Architecture, Fuzhou 350002, Peoples R China
关键词
EXPRESSION ANALYSIS; FLOWER BUDS; FAMILY; GENERATION; EPIPHYTISM; PROTEINS; ESTS;
D O I
10.1038/sdata.2016.83
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] The genome sequence of the orchid Phalaenopsis equestris
    Cai, Jing
    Liu, Xin
    Vanneste, Kevin
    Proost, Sebastian
    Tsai, Wen-Chieh
    Liu, Ke-Wei
    Chen, Li-Jun
    He, Ying
    Xu, Qing
    Bian, Chao
    Zheng, Zhijun
    Sun, Fengming
    Liu, Weiqing
    Hsiao, Yu-Yun
    Pan, Zhao-Jun
    Hsu, Chia-Chi
    Yang, Ya-Ping
    Hsu, Yi-Chin
    Chuang, Yu-Chen
    Dievart, Anne
    Dufayard, Jean-Francois
    Xu, Xun
    Wang, Jun-Yi
    Wang, Jun
    Xiao, Xin-Ju
    Zhao, Xue-Min
    Du, Rong
    Zhang, Guo-Qiang
    Wang, Meina
    Su, Yong-Yu
    Xie, Gao-Chang
    Liu, Guo-Hui
    Li, Li-Qiang
    Huang, Lai-Qiang
    Luo, Yi-Bo
    Chen, Hong-Hwa
    Van de Peer, Yves
    Liu, Zhong-Jian
    NATURE GENETICS, 2015, 47 (01) : 65 - +
  • [2] De Novo Assembly and Developmental Transcriptome Analysis of the Small White Butterfly Pieris rapae
    Qi, Lixing
    Fang, Qi
    Zhao, Lei
    Xia, Hao
    Zhou, Yuxun
    Xiao, Junhua
    Li, Kai
    Ye, Gongyin
    PLOS ONE, 2016, 11 (07):
  • [3] Characterization of Common Carp Transcriptome: Sequencing, De Novo Assembly, Annotation and Comparative Genomics
    Ji, Peifeng
    Liu, Guiming
    Xu, Jian
    Wang, Xumin
    Li, Jiongtang
    Zhao, Zixia
    Zhang, Xiaofeng
    Zhang, Yan
    Xu, Peng
    Sun, Xiaowen
    PLOS ONE, 2012, 7 (04):
  • [4] Parallelization of the Trinity pipeline for de novo transcriptome assembly
    Sachdeva, V.
    Kim, C. S.
    Jordan, K. E.
    Winn, M. D.
    PROCEEDINGS OF 2014 IEEE INTERNATIONAL PARALLEL & DISTRIBUTED PROCESSING SYMPOSIUM WORKSHOPS (IPDPSW), 2014, : 567 - 576
  • [5] Effect of de novo transcriptome assembly on transcript quantification
    Hsieh, Ping-Han
    Oyang, Yen-Jen
    Chen, Chien-Yu
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [6] TransPi-a comprehensive TRanscriptome ANalysiS PIpeline for de novo transcriptome assembly
    Rivera-Vicens, Ramon E.
    Garcia-Escudero, Catalina A.
    Conci, Nicola
    Eitel, Michael
    Woerheide, Gert
    MOLECULAR ECOLOGY RESOURCES, 2022, 22 (05) : 2070 - 2086
  • [7] De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)
    Mamrot, Jared
    Legaie, Roxane
    Ellery, Stacey J.
    Wilson, Trevor
    Seemann, Torsten
    Powell, David R.
    Gardner, David K.
    Walker, David W.
    Temple-Smith, Peter
    Papenfuss, Anthony T.
    Dickinson, Hayley
    SCIENTIFIC REPORTS, 2017, 7
  • [8] Assessing De Novo transcriptome assembly metrics for consistency and utility
    O'Neil, Shawn T.
    Emrich, Scott J.
    BMC GENOMICS, 2013, 14
  • [9] De Novo Transcriptome Assembly of Pummelo and Molecular Marker Development
    Liang, Mei
    Yang, Xiaoming
    Li, Hang
    Su, Shiying
    Yi, Hualin
    Chai, Lijun
    Deng, Xiuxin
    PLOS ONE, 2015, 10 (03):
  • [10] Sequencing, de novo assembly and comparative analysis of Raphanus sativus transcriptome
    Wu, Gang
    Zhang, Libin
    Yin, Yongtai
    Wu, Jiangsheng
    Yu, Longjiang
    Zhou, Yanhong
    Li, Maoteng
    FRONTIERS IN PLANT SCIENCE, 2015, 6