Micropropagation of Actinidia polygama from fruit galls

Callus was induced from the fruit galls of Actinidia polygama on the B5 medium supplemented with NAA, BA and kinetin (1 mg l(-1) each). When the callus was subcultured on the same medium 3 times, shoot primordia and adventitious roots appeared on the surface of the callus. Shoots subcultured on growth regulator free MS medium or supplemented with NAA (1 mg l(-1)) formed roots. Chromosome numbers in the root tip of regenerated plantlets were determined as 2n=58, which was the same as that of the mother plants.