Micropropagation of Actinidia polygama from fruit galls

被引:1
|
作者
Tanaka, H
Shoyama, Y
Sasaki, Y
Sashida, Y
机构
[1] KYUSHU UNIV,FAC PHARMACEUT SCI,HIGASHI KU,FUKUOKA 812,JAPAN
[2] OITA PREFECTURAL FOREST EXPT STN,OITA 87713,JAPAN
[3] TOKYO COLL PHARM,TOKYO 19203,JAPAN
关键词
Actinidiaceae; fruit gall; plantlet; regeneration;
D O I
10.1023/A:1005806607349
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Callus was induced from the fruit galls of Actinidia polygama on the B5 medium supplemented with NAA, BA and kinetin (1 mg l(-1) each). When the callus was subcultured on the same medium 3 times, shoot primordia and adventitious roots appeared on the surface of the callus. Shoots subcultured on growth regulator free MS medium or supplemented with NAA (1 mg l(-1)) formed roots. Chromosome numbers in the root tip of regenerated plantlets were determined as 2n=58, which was the same as that of the mother plants.
引用
收藏
页码:135 / 137
页数:3
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