Eps8 promotes cellular growth of human malignant gliomas

被引：24

作者：

Ding, Xiaofeng ^{[1
]}

Zhou, Fangliang ^{[1
,2
]}

Wang, Fangmei ^{[1
]}

Yang, Zijian ^{[1
]}

Zhou, Chang ^{[1
]}

Zhou, Jianlin ^{[1
]}

Zhang, Bo ^{[1
,2
]}

Yang, Junmei ^{[3
]}

Wang, Guangwei ^{[1
,3
]}

Wei, Zheng ^{[1
]}

Hu, Xiang ^{[1
]}

Xiang, Shuanglin ^{[1
]}

Zhang, Jian ^{[1
]}

机构：

[1] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, State Educ Minist China, Coll Life Sci, Changsha 410081, Hunan, Peoples R China

[2] Hunan Univ Chinese Med, Coll Basic Med Sci, Changsha, Hunan, Peoples R China

[3] Hunan Normal Univ, Sch Med, Changsha 410081, Hunan, Peoples R China

来源：

ONCOLOGY REPORTS | 2013年 / 29卷 / 02期

基金：

中国国家自然科学基金;

关键词：

EGF receptor substrate 8; glioma; cellular growth; lentiviral RNAi system; extracellular signal-regulated protein kinase; phosphorylated serine-threonine protein kinase Akt; beta-catenin; FOCAL ADHESION KINASE; FACTOR RECEPTOR; COLON-CANCER; IN-VIVO; EXPRESSION; PROLIFERATION; CELLS; PHOSPHORYLATION; LOCALIZATION; ACTIVATION;

D O I：

10.3892/or.2012.2160

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Eps8 was initially identified as a substrate of the epidermal growth factor receptor. Overexpression of Eps8 leads to increased mitogenic signaling and malignant transformation. However, little is known concerning the importance of Eps8 in human gliomas In this study, we found that Eps8 was overexpressed in 56.6% of human gliomas (WHO grades III and IV) compared with adjacent normal brain tissues by immunohistochemical analysis. The U251 human glioma cell line stably expressing Eps8 was established by G418 screening, and the ectopic expression of Eps8 enhanced U251 glioma cell growth and survival by cell survival, MTT and liquid colony formation assays. By contrast, the lentiviral expression of Eps8 siRNA in SHG-44 cells resulted in a significant reduction in cellular growth and proliferation. Furthermore, Eps8 modulated the levels of phosphorylated extracellular signal-regulated protein kinase (ERK), phosphorylated serine-threonine protein kinase Akt and beta-catenin expression in glioma cell lines and tissues. These results suggest that Eps8 is overexpressed in human gliomas, and affects glioma cell growth possibly by regulating ERK and Akt/beta-catenin signaling. Therefore, Eps8 may represent a novel potential target in human glioma therapy.

引用

页码：697 / 703

页数：7

共 35 条

[1]

Barker N, 2000, BIOESSAYS, V22, P961

[2]

CASTAGNINO P, 1995, ONCOGENE, V10, P723

[3] Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis [J].

Chen, Huijun ;

Wu, Xufeng ;

Pan, Zhixing K. ;

Huang, Shuang .

CANCER RESEARCH, 2010, 70 (23) :9979-9990

[4] Eps8 decreases chemosensitivity and affects survival of cervical cancer patients [J].

Chen, Yun-Ju ;

Shen, Meng-Ru ;

Chen, Yen-Jen ;

Maa, Ming-Chei ;

Leu, Tzeng-Horng .

MOLECULAR CANCER THERAPEUTICS, 2008, 7 (06) :1376-1385

[5]

EKSTRAND AJ, 1991, CANCER RES, V51, P2164

[6] EPS8, A SUBSTRATE FOR THE EPIDERMAL GROWTH-FACTOR RECEPTOR KINASE, ENHANCES EGF-DEPENDENT MITOGENIC SIGNALS [J].

FAZIOLI, F ;

MINICHIELLO, L ;

MATOSKA, V ;

CASTAGNINO, P ;

MIKI, T ;

WONG, WT ;

DIFIORE, PP .

EMBO JOURNAL, 1993, 12 (10) :3799-3808

[7] Cost of migration: Invasion of malignant gliomas and implications for treatment [J].

Giese, A ;

Bjerkvig, R ;

Berens, ME ;

Westphal, M .

JOURNAL OF CLINICAL ONCOLOGY, 2003, 21 (08) :1624-1636

[8]

Hecquet C, 2002, INVEST OPHTH VIS SCI, V43, P3091

[9] Animal Models of Colitis-Associated Carcinogenesis [J].

Kanneganti, Manasa ;

Mino-Kenudson, Mari ;

Mizoguchi, Emiko .

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2011,

[10] Inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor kinase suppresses glioma proliferation in vitro and in vivo [J].

Liu, Ta-Jen ;

LaFortune, Tiffany ;

Honda, Toshiyuki ;

Ohmori, Osamu ;

Hatakeyama, Shinji ;

Meyer, Thomas ;

Jackson, Dowdy ;

de Groot, John ;

Yung, W. K. Alfred .

MOLECULAR CANCER THERAPEUTICS, 2007, 6 (04) :1357-1367

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