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Chromatographic behavior of selected antibiotic drugs supported by quantitative structure-retention relationships
被引:10
作者:
Szultka-Mlynska, Malgorzata
[1
,2
]
Buszewski, Boguslaw
[1
,2
]
机构:
[1] Nicolaus Copernicus Univ, Dept Environm Chem & Bioanalyt, Fac Chem, Gagarin 7, PL-87100 Torun, Poland
[2] Nicolaus Copernicus Univ, Ctr Modern Interdisciplinary Technol, Wilenska 4, PL-87100 Torun, Poland
关键词:
Antibiotic drugs;
Reversed-phase high performance liquid chromatography (RP HPLC);
Quantitative structure-retention relationships (QSRRs);
Structural descriptors;
Chemically bonded stationary phases;
Retention mechanism;
PERFORMANCE LIQUID-CHROMATOGRAPHY;
BONDED STATIONARY PHASES;
SELECTIVITY;
HPLC;
D O I:
10.1016/j.chroma.2016.11.057
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
As antibiotic drugs have a wide variety of applications, there is a growing demand for their selective determination, a task for which chromatographic methods seem to be appropriate. With this end in view, chromatographic measurements were performed with the use of six different HPLC columns (ACE 5C(18), IAM.PC.DD.2, octadecyl, phenyl, alkylamide and cholesteryl-alkylamide) to determine the logk(w) of selected antibiotic drugs (amoxicillin, cefatoxime, ciprofloxacin, fluconazole, gentamicin, clindamycin, linezolid and metronidazole). The retention behavior of the analytes was investigated as a function of different binary hydro-organic mobile phases containing 10-45% (v/v) acetonitrile. The studied compounds were separated under isocratic conditions. The best results of separation of the tested biologically active compounds were obtained on the commercially available C18 column. Special attention was dedicated to the study of interactions among the stationary phase, mobile phase and the analytes. Another goal was to selecting the best column for separation of the tested biologically active compounds. Finally, QSRR models together with stationary phase characterization provided reliable information on the properties and characteristics of studied columns. (C) 2016 Elsevier B.V. All rights reserved.
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页码:50 / 59
页数:10
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