Antimicrobial efficacy of the alkaloid harmaline alone and in combination with chlorhexidine digluconate against clinical isolates of Staphylococcus aureus grown in planktonic and biofilm cultures

被引:33
作者
Xing, M. [1 ]
Shen, F. [1 ]
Liu, L. [1 ]
Chen, Z. [2 ]
Guo, N. [3 ]
Wang, X. [1 ]
Wang, W. [1 ]
Zhang, K. [4 ]
Wu, X. [1 ]
Wang, X. [1 ]
Li, Y. [1 ]
Sun, S. [5 ]
Yu, L. [1 ,6 ]
机构
[1] Jilin Univ, Coll Anim Sci & Vet Med, Inst Zoonosis, Key Lab Zoonosis Res,Minist Educ, Changchun 130062, Peoples R China
[2] Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing, Heilongjiang, Peoples R China
[3] Jilin Univ, Dept Food Qual & Safety, Coll Quartermaster Technol, Changchun 130062, Peoples R China
[4] Jilin Univ, Dept Infect Dis, Hosp 1, Changchun 130062, Peoples R China
[5] Inner Mongolia Univ Nationalities, Anim Sci & Technol Coll, Tongliao, Peoples R China
[6] Chinese Ctr Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China
关键词
antimicrobial activity; biofilm; chlorhexidine digluconate; harmaline; synergy; BETA-CARBOLINE ALKALOIDS; PEGANUM-HARMALA; SYNERGISTIC ACTIVITY; EUCALYPTUS OIL; L; SEEDS; INFECTIONS; INHIBITION; GUIDELINES; EXCHANGE; HARMINE;
D O I
10.1111/j.1472-765X.2012.03233.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To investigate the antimicrobial efficacy of an alkaloid, harmaline alone and in combination with chlorhexidine digluconate (CHG) against clinical isolates of Staphylococcus aureus (S. aureus) grown in planktonic and biofilm cultures. Methods: Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined for each micro-organism grown in suspension and in biofilm using microbroth dilution method. Chequerboard assays were used to determine synergistic, indifferent or antagonistic interactions between harmaline and CHG, and the some of results were verified by confocal laser scanning microscopy. Results: Harmaline and CHG showed effective antimicrobial activity against suspensions and biofilm cultures of S. aureus, respectively. As determined by fractional inhibitory concentration index (FICI), synergistic antimicrobial effects between harmaline and CHG were observed in nine and 11 of the 13 S. aureus strains when in suspension and in biofilm, respectively. FICI values were from 0 375 to 1 25 when in suspension and from 0 25 to 1 25 when in biofilm. Conclusions: Synergistic activity of harmaline and CHG against clinical isolates of S. aureus (in suspension and in biofilm) was observed in vitro. Significance and Impact of the Study: This study might provide alternative methods to overcome the problem of drug-resistance of S. aureus both in suspension and in biofilm.
引用
收藏
页码:475 / 482
页数:8
相关论文
共 32 条
[31]  
Sobhani AM, 2002, J PHARM PHARM SCI, V5, P19
[32]   INHIBITION OF NA+-CA2+ EXCHANGE MECHANISM IN CARDIAC SARCOLEMMAL VESICLES BY HARMALINE [J].
SULEIMAN, MS ;
REEVES, JP .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1987, 88 (01) :197-200