Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation

被引:13
作者
Fang, Weiping [1 ]
Xie, Deyi [1 ]
Zhu, Heqin [2 ]
Li, Wu [1 ]
Xu, Zhenzhen [2 ]
Yang, Lirong [3 ]
Li, Zhifang [2 ]
Sun, Li [4 ]
Wang, Jinxia [5 ]
Nie, Lihong [1 ]
Tang, Zhongjie [1 ]
Lv, Shuping [1 ]
Zhao, Fu'an [1 ]
Sun, Yao [1 ]
Zhao, Yuanming [1 ]
Hou, Jianan [1 ]
Yang, Xiaojie [1 ]
机构
[1] Henan Acad Agr Sci, Econ Crop Res Inst, Zhengzhou 450002, Peoples R China
[2] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, South Korea
[3] Henan Acad Agr Sci, Plant Protect Res Inst, Zhengzhou 450002, Peoples R China
[4] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA
[5] Henan Agr Univ, Agron Coll, Dept Crop Biotechnol, Zhengzhou 450002, Peoples R China
关键词
cotton; defense response; fungus; iTRAQ; SALICYLIC-ACID; TRANSCRIPTIONAL ANALYSIS; DEFENSE RESPONSES; COTTON; RESISTANCE; PROTEIN; WILT; EXPRESSION; GENOME; IDENTIFICATION;
D O I
10.3390/ijms161025121
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Verticillium wilt is threatening cotton productivity globally. This disease is caused by soil-borne Verticillium dahliae which directly infects cotton roots, and exclusively colonizes and occludes xylem vessels, finally resulting in necrosis, defoliation, and most severely, plant death. For the first time, iTRAQ (isobaric tags for relative and absolute quantification) was applied to screen the differentially expressed proteins of Gossypium thurberi inoculated with V. dahliae. A total of 6533 proteins were identified from the roots of G. thurberi after inoculation with V. dahliae, and 396 showed up- and 279 down-regulated in comparison to a mock-inoculated roots. Of these identified proteins, the main functional groups were those involved in cell wall organization and reinforcement, disease-resistant chemicals of secondary metabolism, phytohormone signaling, pathogenesis-related proteins, and disease-resistant proteins. Physiological and biochemical analysis showed that peroxidase activity, which promotes the biosynthesis and accumulation of lignin, was induced early in the hypocotyl after inoculation with V. dahliae. Similarly, salicylic acid also accumulated significantly in hypocotyl of the seedlings after inoculation. These findings provide an important knowledge of the molecular events and regulatory networks occurring during G. thurberi-V. dahliae interaction, which may provide a foundation for breeding disease-resistance in cotton.
引用
收藏
页码:25121 / 25140
页数:20
相关论文
共 50 条
[41]   Dynamic characteristics and functional analysis provide new insights into long non-coding RNA responsive to Verticillium dahliae infection in Gossypium hirsutum [J].
Guoning Wang ;
Xingfen Wang ;
Yan Zhang ;
Jun Yang ;
Zhikun Li ;
Lizhu Wu ;
Jinhua Wu ;
Nan Wu ;
Lixia Liu ;
Zhengwen Liu ;
Man Zhang ;
Liqiang Wu ;
Guiyin Zhang ;
Zhiying Ma .
BMC Plant Biology, 21
[42]   Genome-Wide Identification of Papain-Like Cysteine Proteases in Gossypium hirsutum and Functional Characterization in Response to Verticillium dahliae [J].
Zhang, Shuqin ;
Xu, Zhongping ;
Sun, Heng ;
Sun, Longqing ;
Shaban, Muhammad ;
Yang, Xiyan ;
Zhu, Longfu .
FRONTIERS IN PLANT SCIENCE, 2019, 10
[43]   THE DETERMINATION OF RELATIONSHIP BETWEEN VERTICILLIUM WILT (Verticillium dahliae Kleb.) AND EARLY MATURITY IN COTTON (Gossypium hirsutum L.) [J].
Erdogan, Oktay ;
Ozbek, Nedim ;
Unay, Aydin ;
Gore, M. Erhan .
TURKISH JOURNAL OF FIELD CROPS, 2013, 18 (01) :8-12
[44]   DETERMINATION OF SUSCEPTIBILITY OF SOME COTTON GENOTYPES (Gossypium spp.) AGAINST Verticillium dahliae KLEB [J].
Celik, Sadettin ;
Erdogan, Oktay ;
Bardak, Adem .
FRESENIUS ENVIRONMENTAL BULLETIN, 2022, 31 (03) :2409-+
[45]   Identification of high-affinity nicotinic acid transporter genes from Verticillium dahliae and functional analysis based on HIGS technology [J].
Wang, Yuan ;
Kamau, Stephen ;
Song, Shenglong ;
Zhang, Yong ;
Zhang, Xinyu .
JOURNAL OF COTTON RESEARCH, 2025, 8 (01)
[46]   Molecular cloning and functional analysis of GbRVd, a gene in Gossypium barbadense that plays an important role in conferring resistance to Verticillium wilt [J].
Yang, Jun ;
Ma, Qing ;
Zhang, Yan ;
Wang, Xingfen ;
Zhang, Guiyin ;
Ma, Zhiying .
GENE, 2016, 575 (02) :687-694
[47]   RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton [J].
Dong, Qi ;
Magwanga, Richard Odongo ;
Cai, Xiaoyan ;
Lu, Pu ;
Kirungu, Joy Nyangasi ;
Zhou, Zhongli ;
Wang, Xingfen ;
Wang, Xingxing ;
Xu, Yanchao ;
Hou, Yuqing ;
Wang, Kunbo ;
Peng, Renhai ;
Ma, Zhiying ;
Liu, Fang .
GENES, 2019, 10 (02)
[48]   GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism [J].
Xiao, Shenghua ;
Ming, Yuqing ;
Hu, Qin ;
Ye, Zhengxiu ;
Si, Huan ;
Liu, Shiming ;
Zhang, Xiaojun ;
Wang, Weiran ;
Yu, Yu ;
Kong, Jie ;
Klosterman, Steven J. ;
Lindsey, Keith ;
Zhang, Xianlong ;
Aierxi, Alifu ;
Zhu, Longfu .
PLANT BIOTECHNOLOGY JOURNAL, 2023, :961-978
[49]   A novel Gossypium barbadense ERF transcription factor, GbERFb, regulation host response and resistance to Verticillium dahliae in tobacco [J].
Liu, Jianguang ;
Wang, Yongqiang ;
Zhao, Guiyuan ;
Zhao, Junli ;
Du, Haiying ;
He, Xiaoliang ;
Zhang, Hanshuang .
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2017, 23 (01) :125-134
[50]   Proteomic analysis of grapevine stem in response to Xylella fastidiosa inoculation [J].
Yang, Litao ;
Lin, Hong ;
Takahashi, Yuri ;
Chen, Feng ;
Walker, M. Andrew ;
Civerolo, Edwin L. .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2011, 75 (03) :90-99