Isobavachalcone Induces Multiple Cell Death in Human Triple-Negative Breast Cancer MDA-MB-231 Cells

被引:14
作者
Wu, Cheng-Zhu [1 ,2 ]
Gao, Mei-Jia [1 ]
Chen, Jie [1 ]
Sun, Xiao-Long [1 ]
Zhang, Ke-Yi [1 ]
Dai, Yi-Qun [1 ]
Ma, Tao [1 ]
Li, Hong-Mei [1 ,2 ]
Zhang, Yu-Xin [2 ,3 ]
机构
[1] Bengbu Med Coll, Sch Pharm, 2600 Donghai Rd, Bengbu 233030, Peoples R China
[2] Anhui Prov Biochem Pharmaceut Engn Technol Res Ct, Bengbu 233030, Peoples R China
[3] Bengbu Med Coll, Sch Lab Med, 2600 Donghai Rd, Bengbu 233030, Peoples R China
关键词
TNBC; natural product; isobavachalcone; cell death; necroptosis; RIP3; DRUG-RESISTANCE; APOPTOSIS; NECROPTOSIS; FLAVONOIDS; INDUCTION; RIP3;
D O I
10.3390/molecules27206787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Standardized treatment guidelines and effective drugs are not available for human triple-negative breast cancer (TNBC). Many efforts have recently been exerted to investigate the efficacy of natural compounds as anticancer agents owing to their low toxicity. However, no study has examined the effects of isobavachalcone (IBC) on the programmed cell death (PCD) of human triple-negative breast MDA-MB-231 cancer cells. In this study, IBC substantially inhibited the proliferation of MDA-MB-231 cells in concentration- and time-dependent manners. In addition, we found that IBC induced multiple cell death processes, such as apoptosis, necroptosis, and autophagy in MDA-MB-231 cells. The initial mechanism of IBC-mediated cell death in MDA-MB-231 cells involves the downregulation of Akt and p-Akt-473, an increase in the Bax/Bcl-2 ratio, and cleaved caspases-3 induced apoptosis; the upregulation of RIP3, p-RIP3 and MLKL induced necroptosis; as well as a simultaneous increase in LC3-II/I ratio induced autophagy. In addition, we observed that IBC induced mitochondrial dysfunction, thereby decreasing cellular ATP levels and increasing reactive oxygen species accumulation to induce PCD. These results suggest that IBC is a promising lead compound with anti-TNBC activity.
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页数:16
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