Bioelectrocatalytical glucose oxidation with phenoxazine modified glucose oxidase

被引：32

作者：

Krikstopaitis, K ^{[1
]}

Kulys, J ^{[1
]}

Tetianec, L ^{[1
]}

机构：

[1] Inst Biochem, Lab Enzyme Chem, LT-08662 Vilnius, Lithuania

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2004年 / 6卷 / 04期

关键词：

glucose oxidase; glucose; phenoxazine; electron transfer;

D O I：

10.1016/j.elecom.2004.01.011

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Phenoxazine mediator bearing a carboxylate group was covalently attached to glucose oxidase by a carbodiimide coupling procedure. Spectral measurements showed that about 20 molecules of the mediator were covalently bonded to enzyme. The modified enzyme saved initial activity. Quenching of tryptophan fluorescence confirmed that mediator was covalently attached to glucose oxidase. The attached mediator mediated efficiently the electron transfer during reoxidation of the modified glucose oxidase in the presence of glucose. It was suggested that mediated electron transfer proceeded by intra- and intermolecular mechanism. Biosensor based on the modified glucose oxidase with phenoxazine was developed and bioelectrocatalytical properties determined. The electrode was poised at 0.4 V vs. SCE and linear response was obtained over the range of 3-8 mM glucose. (C) 2004 Published by Elsevier B.V.

引用

页码：331 / 336

页数：6

共 17 条

[1]

BADDIA A, 1993, J AM CHEM SOC, V115, P7053

[2] Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface [J].

Ban, K ;

Ueki, T ;

Tamada, Y ;

Saito, T ;

Imabayashi, S ;

Watanabe, M .

ELECTROCHEMISTRY COMMUNICATIONS, 2001, 3 (11) :649-653

[3] Modification of glucose oxidase by the covalent attachment of a tetrathiafulvalene derivative [J].

Bartlett, PN ;

Booth, S ;

Caruana, DJ ;

Kilburn, JD ;

Santamaria, C .

ANALYTICAL CHEMISTRY, 1997, 69 (04) :734-742

[4] Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose [J].

Battaglini, F ;

Bartlett, PN ;

Wang, JH .

ANALYTICAL CHEMISTRY, 2000, 72 (03) :502-509

[5] FERROCENE-MEDIATED ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].

CASS, AEG ;

DAVIS, G ;

FRANCIS, GD ;

HILL, HAO ;

ASTON, WJ ;

HIGGINS, IJ ;

PLOTKIN, EV ;

SCOTT, LDL ;

TURNER, APF .

ANALYTICAL CHEMISTRY, 1984, 56 (04) :667-671

[6] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .2. METHODS FOR BONDING ELECTRON-TRANSFER RELAYS TO GLUCOSE-OXIDASE AND D-AMINO-ACID OXIDASE [J].

DEGANI, Y ;

HELLER, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (08) :2615-2620

[7] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .1. ELECTRON-TRANSFER FROM GLUCOSE-OXIDASE TO METAL-ELECTRODES VIA ELECTRON RELAYS, BOUND COVALENTLY TO THE ENZYME [J].

DEGANI, Y ;

HELLER, A .

JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (06) :1285-1289

[8] *ZWISCHENMOLEKULARE ENERGIEWANDERUNG UND FLUORESZENZ [J].

FORSTER, T .

ANNALEN DER PHYSIK, 1948, 2 (1-2) :55-75

[9] CRYSTAL-STRUCTURE OF GLUCOSE-OXIDASE FROM ASPERGILLUS-NIGER REFINED AT 2 .3 ANGSTROM RESOLUTION [J].

HECHT, HJ ;

KALISZ, HM ;

HENDLE, J ;

SCHMID, RD ;

SCHOMBURG, D .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 229 (01) :153-172

[10] ELECTRICAL WIRING OF REDOX ENZYMES [J].

HELLER, A .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (05) :128-134

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