Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda

被引:29
作者
Duan, Yafei [1 ,2 ]
Liu, Ping [1 ]
Li, Jitao [1 ]
Li, Jian [1 ]
Chen, Ping [1 ]
机构
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
[2] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词
Exopalaemon carinicauda; Hemocytes; Expressed sequence tags; Immune-related gene; FRESH-WATER CRAYFISH; BLACK TIGER SHRIMP; MOLECULAR-CLONING; LITOPENAEUS-VANNAMEI; ANTIMICROBIAL ACTIVITY; SERINE-PROTEASE; CHINESE SHRIMP; KURUMA PRAWN; CRUSTIN-LIKE; ANTILIPOPOLYSACCHARIDE FACTOR;
D O I
10.1016/j.fsi.2012.10.026
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The ridgetail white prawn Exopalaemon carinicauda is one of the most important commercial species in eastern China. However, little information of immune genes in E. carinicauda has been reported. To identify distinctive genes associated with immunity, an expressed sequence tag (EST) library was constructed from hemocytes of E. carinicauda. A total of 3411 clones were sequenced, yielding 2853 ESTs and the average sequence length is 436 bp. The cluster and assembly analysis yielded 1053 unique sequences including 329 contigs and 724 singletons. Blast analysis identified 593 (56.3%) of the unique sequences as orthologs of genes from other organisms (E-value < 1e-5). Based on the COG and Gene Ontology (GO), 593 unique sequences were classified. Through comparison with previous studies, 153 genes assembled from 367 ESTs have been identified as possibly involved in defense or immune functions. These genes are categorized into seven categories according to their putative functions in shrimp immune system: antimicrobial peptides, prophenoloxidase activating system, antioxidant defense systems, chaperone proteins, clottable proteins, pattern recognition receptors and other immune-related genes. According to EST abundance, the major immune-related genes were thioredoxin (141, 4.94% of all ESTs) and calmodulin (14, 0.49% of all ESTs). The EST sequences of E. carinicauda hemocytes provide important information of the immune system and lay the groundwork for development of molecular markers related to disease resistance in prawn species. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:173 / 182
页数:10
相关论文
共 77 条
[21]   Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas [J].
Gueguen, Y ;
Cadoret, JP ;
Flament, D ;
Barreau-Roumiguière, C ;
Girardot, AL ;
Garnier, J ;
Hoareau, A ;
Bachère, E ;
Escoubas, JM .
GENE, 2003, 303 :139-145
[22]   The serine protease motif of Pic mediates a dose-dependent mucolytic activity after binding to sugar constituents of the mucin substrate [J].
Gutierrez-Jimenez, Javier ;
Arciniega, Ivonne ;
Navarro-Garcia, Fernando .
MICROBIAL PATHOGENESIS, 2008, 45 (02) :115-123
[23]   Molecular characterization of a Ran isoform gene up-regulated in shrimp immunity [J].
Han, Fang ;
Wang, Xiaoqing ;
Wang, Zhiyong .
GENE, 2012, 495 (01) :65-71
[24]  
[韩俊英 HAN Junying], 2011, [水产学报, Journal of Fisheries of China], V35, P1130
[25]   Cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria [J].
Hauton, C ;
Brockton, V ;
Smith, VJ .
MOLECULAR IMMUNOLOGY, 2006, 43 (09) :1490-1496
[26]   Purification and characterization of chymotrypsin from Penaeus vannamei (Crustacea: Decapoda) [J].
Hernandez-Cortes, P ;
Whitaker, JR ;
Garcia-Carreno, FL .
JOURNAL OF FOOD BIOCHEMISTRY, 1997, 21 (06) :497-514
[27]  
Hernandez-Lopez J, 1996, COMP BIOCH PHYSL C, V113, P116
[28]   Penaeus monodon SERPIN, PmSERPIN6, is implicated in the shrimp innate immunity [J].
Homvises, Teerada ;
Tassanakajon, Anchalee ;
Somboonwiwat, Kunlaya .
FISH & SHELLFISH IMMUNOLOGY, 2010, 29 (05) :890-898
[29]   Antibacterial peptides in hemocytes and hematopoietic tissue from freshwater crayfish Pacifastacus leniusculus:: Characterization and expression pattern [J].
Jiravanichpaisal, Pikul ;
Lee, So Young ;
Kim, Young-A ;
Andren, Tove ;
Soderhall, Irene .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2007, 31 (05) :441-455
[30]   Serine proteinase inhibitors in arthropod immunity [J].
Kanost, MR .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1999, 23 (4-5) :291-301