Molecular design of aquaporin-1 water channel as revealed by electron crystallography

被引：104

作者：

Li, HL ^{[1
]}

Lee, S ^{[1
]}

Jap, BK ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV LIFE SCI,BERKELEY,CA 94720

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 04期

关键词：

D O I：

10.1038/nsb0497-263

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The electron crystallographic structure of the aquaporin-1 water channel, determined at similar to 6 Angstrom, reveals that the protein has six transmembrane alpha-helices forming a trapezoid-like cylinder. There is a branched rod-like structure within the cylinder that traverses the membrane and likely contains at least one alpha-helix.

引用

页码：263 / 265

页数：3

共 23 条

[1]

AGRE P, 1993, AM J PHYSIOL, V265, pF463

[2] AQUAPORIN WATER CHANNELS - UNANSWERED QUESTIONS AND UNRESOLVED CONTROVERSIES [J].

AGRE, P ;

BROWN, D ;

NIELSEN, S .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (04) :472-483

[3]

[Anonymous], ACTA CRYSTALLOGR

[4] MRC image processing programs [J].

Crowther, RA ;

Henderson, R ;

Smith, JM .

JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) :9-16

[5] SPOT-SCAN IMAGING IN TRANSMISSION ELECTRON-MICROSCOPY [J].

DOWNING, KH .

SCIENCE, 1991, 251 (4989) :53-59

[6] THE MAJOR INTRINSIC PROTEIN (MIP) OF THE BOVINE LENS FIBER MEMBRANE - CHARACTERIZATION AND STRUCTURE BASED ON CDNA CLONING [J].

GORIN, MB ;

YANCEY, SB ;

CLINE, J ;

REVEL, JP ;

HORWITZ, J .

CELL, 1984, 39 (01) :49-59

[7] 3-DIMENSIONAL MODEL OF PURPLE MEMBRANE OBTAINED BY ELECTRON-MICROSCOPY [J].

HENDERSON, R ;

UNWIN, PNT .

NATURE, 1975, 257 (5521) :28-32

[8] STRUCTURE OF PURPLE MEMBRANE FROM HALOBACTERIUM-HALOBIUM - RECORDING, MEASUREMENT AND EVALUATION OF ELECTRON-MICROGRAPHS AT 3.5 A RESOLUTION [J].

HENDERSON, R ;

BALDWIN, JM ;

DOWNING, KH ;

LEPAULT, J ;

ZEMLIN, F .

ULTRAMICROSCOPY, 1986, 19 (02) :147-178

[9] STRUCTURE OF THE OSMO-REGULATED H2O-CHANNEL, AQP-CHIP, IN PROJECTION AT 3.5 ANGSTROM RESOLUTION [J].

JAP, BK ;

LI, HL .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 251 (03) :413-420

[10]

JUNG JS, 1994, J BIOL CHEM, V269, P14648

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