Mycobacterium tuberculosis H37Rv LpqG Protein Peptides Can Inhibit Mycobacterial Entry through Specific Interactions

被引:7
作者
David Sanchez-Barinas, Christian [1 ,2 ]
Ocampo, Marisol [1 ,2 ]
Vanegas, Magnolia [1 ,2 ]
Johana Castaneda-Ramirez, Jeimmy [1 ,2 ]
Alfonso Patarroyo, Manuel [1 ,2 ]
Elkin Patarroyo, Manuel [1 ,3 ]
机构
[1] Fdn Inst Inmunol Colombia FIDIC, Carrera 50 26-20, Bogota 111321, Colombia
[2] Univ Rosario, Sch Med & Hlth Sci, Basic Sci Dept, Carrera 24 63C-69, Bogota 111321, Colombia
[3] Univ Nacl Colombia, Fac Med, Pathol Dept, Carrera 45 26-85, Bogota 111321, Colombia
来源
MOLECULES | 2018年 / 23卷 / 03期
关键词
LpqG; lipoprotein; Mycobacterium tuberculosis; vaccine; synthetic peptide; Rv3623; mycobacterial entry inhibition; PREDICTING SUBCELLULAR-LOCALIZATION; ACTIVITY BINDING PEPTIDES; SIGNAL PEPTIDES; SECONDARY STRUCTURE; WEB; MEMBRANE; VACCINE; FRACTIONATION; LIPOPROTEINS; IMMUNITY;
D O I
10.3390/molecules23030526
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mycobacterium tuberculosis is the causative agent of tuberculosis, a disease causing major mortality worldwide. As part of a systematic methodology for studying M. tuberculosis surface proteins which might be involved in host-pathogen interactions, our group found that LpqG surface protein (Rv3623) found in M. tuberculosis complex strains was located on the mycobacterial envelope and that peptide 16661 ((21)SGCDSHNSGSLGADPRQVTVY(40)) had high specific binding to U937 monocyte-derived macrophages and inhibited mycobacterial entry to such cells in a concentration-dependent way. A region having high specific binding to A549 alveolar epithelial cells was found which had low mycobacterial entry inhibition. As suggested in previous studies, relevant sequences in the host-pathogen interaction do not induce an immune response and peptides characterised as HABPs are poorly recognised by sera from individuals regardless of whether they have been in contact with M. tuberculosis. Our approach to designing a synthetic, multi-epitope anti-tuberculosis vaccine has been based on identifying sequences involved in different proteins' mycobacteria-target cell interaction and modifying their sequence to improve their immunogenic characteristics, meaning that peptide 16661 sequence should be considered in such design.
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页数:16
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