Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system

被引：3

作者：

da Silva Junior, Haroldo Cid ^{[1
,4
]}

da Silva e Mouta Junior, Sergio ^{[4
]}

Lima de Mendonca, Marcos Cesar ^{[1
]}

de Souza Pereira, Mirian Claudia ^{[3
]}

Nogueira, Alanderson da Rocha ^{[3
]}

Borges de Azevedo, Maria Luiza ^{[2
]}

Gagliardi Leite, Jose Paulo ^{[1
]}

Baroni de Moraes, Marcia Terezinha ^{[4
]}

机构：

[1] Inst Oswaldo Cruz, Lab Virol Comparada & Ambiental, Fdn Oswaldo Cruz, BR-21040360 Rio De Janeiro, RJ, Brazil

[2] Lab Tecnol Recombinante, Biomanguinhos Fdn Oswaldo Cruz, Rio De Janeiro, RJ, Brazil

[3] Inst Oswaldo Cruz, Lab Ultra Estr Celular, Fdn Oswaldo Cruz, BR-21040360 Rio De Janeiro, RJ, Brazil

[4] Inst Oswaldo Cruz, Fdn Oswaldo Cruz, Lab Virol Mol, BR-21040360 Rio De Janeiro, RJ, Brazil

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 09期

关键词：

Rotavirus A; VP6; protein; HEK293-T cells; Sf9; cells; Diagnosis; Vaccine; VIRUS-LIKE PARTICLES; MAMMALIAN-CELLS; VACCINE;

D O I：

10.1007/s10529-012-0946-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.

引用

页码：1623 / 1627

页数：5

共 2 条

[1] Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system
Haroldo Cid da Silva Junior
Sérgio da Silva e Mouta Junior
Marcos César Lima de Mendonça
Mirian Claudia de Souza Pereira
Alanderson da Rocha Nogueira
Maria Luiza Borges de Azevedo
José Paulo Gagliardi Leite
Márcia Terezinha Baroni de Moraes
Biotechnology Letters, 2012, 34 : 1623 - 1627
[2] GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system
Zhang, Ge
Liu, Yan
Yu, Liwei
Sun, Lina
ONCOLOGY REPORTS, 2012, 28 (05) : 1673 - 1680

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