The Combination of Vascular Endothelial Growth Factor and Stromal Cell-Derived Factor Induces Superior Angiogenic Sprouting by Outgrowth Endothelial Cells

被引:12
作者
Anderson, Erin M. [1 ,2 ]
Mooney, David J. [1 ,2 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Cambridge, MA 02138 USA
基金
美国国家卫生研究院;
关键词
Angiogenesis; Endothelial progenitor cells; Therapeutic neovascularization; Vascular endothelial growth factor; Stromal cell-derived factor; PROGENITOR CELLS; CHEMOKINE SDF-1; FACTOR-I; NEOVASCULARIZATION; RECRUITMENT; FACTOR-1-ALPHA; MOBILIZATION; ISCHEMIA; DISEASE; CXCR4;
D O I
10.1159/000382129
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Endothelial progenitor cells are being broadly explored for the treatment of ischemic cardiovascular diseases, but their response to molecules commonly used to promote the growth of new blood vessels has not been fully characterized. In this study, angiogenic sprout formation in a 3-dimensional, in vitro model by one type of endothelial progenitor, outgrowth endothelial cells (OECs), was characterized in response to exposure to stromal cell-derived factor (SDF) and vascular endothelial growth factor (VEGF) and then compared to mature endothelial cells. Exposure to SDF alone did not increase angiogenic sprouting in comparison to control media, while a combination of VEGF and SDF demonstrated greater potency than VEGF alone for all cell types. Together, VEGF and SDF reduced the sprout initiation time and maintained sprouting levels over time. In direct competition with mature endothelial cells, OECs preferentially localized to the tip cell position, suggesting an enhanced sprouting potential. Overall, these results reveal the impact of the combination of VEGF and SDF on endothelial cell sprouting, and support the enhanced potential of OECs, as opposed to mature endothelial cells, for treating ischemic diseases. (C) 2015 S. Karger AG, Basel
引用
收藏
页码:62 / 69
页数:8
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