Evaluation of DNA Damage and Expressions of DNA Repair Gene in Occupationally Lead Exposed Workers (Jodhpur, India)

Occupational exposure to lead (Pb) remains a significant concern for worker's health working in different factories. There are many discrepancies among the results regarding the studies of genotoxicity of Pb. The present study aimed to evaluate DNA damage and expressions of DNA repair genes (OGG1, XRCC1, and XPD) in occupationally Pb-exposed workers of Jodhpur, India. The study consisted of 100 occupationally Pb-exposed workers and 100 controls (non-exposed) with no history of occupational exposure. Pb levels were determined by atomic absorption spectrophotometry, serum 8-hydroxy-2-deoxyguanosine (8-OHdG) concentrations were measured by ELISA, and expressions of DNA repair genes (OGG1, XRCC1, and XPD) were estimated by RT-PCR. The results indicated significantly higher levels of Pb in the exposed group as compared with the non-exposed group (p < 0.0001). Serum 8-OHdG concentrations were significantly higher (p < 0.0083), and all three DNA repair genes were significantly downregulated (fold change: OGG1, 0.188; XRCC1, 0.125; XPD, 0.133) in the Pb-exposed group as compared with the non-exposed. In conclusion, the study findings suggest that Pb exposure is associated with increased DNA damage and reduced DNA repair capacity, which may lead to serious health issues in occupationally Pb-exposed workers.