Cytochrome c heme lyase can mature a fusion peptide composed of the amino-terminal residues of horse cytochrome c

被引：10

作者：

Asher, Wesley B. ^{[1
]}

Bren, Kara L. ^{[1
]}

机构：

[1] Univ Rochester, Dept Chem, Rochester, NY 14627 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 67期

基金：

美国国家科学基金会;

关键词：

ESCHERICHIA-COLI; PDZ DOMAIN; BIOGENESIS; EXPRESSION; PROTEIN; MECHANISM; SYSTEM; TAG;

D O I：

10.1039/c2cc31112g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It is shown that cytochrome c heme lyase (CCHL) attaches heme covalently to peptides composed of the N-terminal segment of cyt c fused to a non-heme containing protein, lending insight into the substrate specificity of CCHL and providing a new route to artificial heme proteins.

引用

收藏

页码：8344 / 8346

页数：3

相关论文

共 25 条

[1] Cytochrome c biogenesis in mitochondria - Systems III and V [J].

Allen, James W. A. .

FEBS JOURNAL, 2011, 278 (22) :4198-4216

[2] What is the substrate specificity of the System I cytochrome c biogenesis apparatus? [J].

Allen, JWA ;

Ferguson, SJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2006, 34 :150-151

[3] A cytochrome b562 variant with a c-type cytochrome CXXCH heme-binding motif as a probe of the Escherichia coli cytochrome c maturation system [J].

Allen, JWA ;

Barker, PD ;

Ferguson, SJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (52) :52075-52083

[4] A heme fusion tag for protein affinity purification and quantification [J].

Asher, Wesley B. ;

Bren, Kara L. .

PROTEIN SCIENCE, 2010, 19 (10) :1830-1839

[5] Heme ladder, a direct molecular weight marker for immunoblot analysis [J].

Bischof, Daniela F. ;

Hardmeier, Sarah ;

Fairhead, Michael ;

Ihssen, Julian ;

Thoeny-Meyer, Linda .

ANALYTICAL BIOCHEMISTRY, 2011, 409 (02) :213-219

[6] The chemistry and biochemistry of heme c: functional bases for covalent attachment [J].

Bowman, Sarah E. J. ;

Bren, Kara L. .

NATURAL PRODUCT REPORTS, 2008, 25 (06) :1118-1130

[7] A heme tag for in vivo synthesis of artificial cytochromes [J].

Braun, M ;

Rubio, IG ;

Thöny-Meyer, L .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 67 (02) :234-239

[8] Biosynthesis of artificial microperoxidases by exploiting the secretion and cytochrome c maturation apparatuses of Escherichia coli [J].

Braun, M ;

Thöny-Meyer, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (35) :12830-12835

[9] A conserved folding mechanism for PDZ domains [J].

Chi, Celestine N. ;

Gianni, Stefano ;

Calosci, Nicoletta ;

Travaglini-Allocatelli, Carlo ;

Engstrom, Ake ;

Jemth, Per .

FEBS LETTERS, 2007, 581 (06) :1109-1113

[10] THE RAT-BRAIN POSTSYNAPTIC DENSITY FRACTION CONTAINS A HOMOLOG OF THE DROSOPHILA DISKS-LARGE TUMOR SUPPRESSOR PROTEIN [J].

CHO, KO ;

HUNT, CA ;

KENNEDY, MB .

NEURON, 1992, 9 (05) :929-942