Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

被引：20

作者：

Gao, Qiang ^{[1
]}

Yang, Hao ^{[2
]}

Wang, Chi ^{[2
]}

Xie, Xin-Ying ^{[2
]}

Liu, Kai-Xuan ^{[2
]}

Lin, Ying ^{[2
]}

Han, Shuang-Yan ^{[2
]}

Zhu, Mingjun ^{[2
]}

Neureiter, Markus ^{[3
]}

Lin, Yina ^{[2
]}

Ye, Jian-Wen ^{[2
]}

机构：

[1] Qinghai Univ, Key Lab Plateau Ecol & Agr, Xining, QH, Peoples R China

[2] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou, Peoples R China

[3] Univ Nat Resources & Life Sci, Inst Environm Biotechnol, Dept Agrobiotechnol, Tulln, Austria

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2022年 / 10卷

基金：

中国国家自然科学基金;

关键词：

polyhydroxyalkanoates; synthetic pathway; metabolic engineering; PHA synthase; microbial production; ESCHERICHIA-COLI; PHA SYNTHASE; RALSTONIA-EUTROPHA; PSEUDOMONAS-PUTIDA; BACTERIAL POLYHYDROXYALKANOATES; ENHANCED PRODUCTION; BIOSYNTHESIS; HALOMONAS; POLY(3-HYDROXYBUTYRATE); ENZYME;

D O I：

10.3389/fbioe.2022.966598

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the rapid development of synthetic biology, a variety of biopolymers can be obtained by recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one with promising material properties, such as biodegradability and biocompatibility against the petrol-based plastics. This study reviews the recent studies focusing on the microbial synthesis of PHA, including chassis engineering, pathways engineering for various substrates utilization and PHA monomer synthesis, and PHA synthase modification. In particular, advances in metabolic engineering of dominant workhorses, for example Halomonas, Ralstonia eutropha, Escherichia coli and Pseudomonas, with outstanding PHA accumulation capability, were summarized and discussed, providing a full landscape of diverse PHA biosynthesis. Meanwhile, we also introduced the recent efforts focusing on structural analysis and mutagenesis of PHA synthase, which significantly determines the polymerization activity of varied monomer structures and PHA molecular weight. Besides, perspectives and solutions were thus proposed for achieving scale-up PHA of low cost with customized material property in the coming future.

引用

页数：10

共 115 条

[1] Polyhydroxyalkanoates (PHA) from Halomonas pacifica ASL10 and Halomonas salifodiane ASL11 isolated from Mariout salt lakes [J].

Abd El-malek, Fady ;

Farag, Aida ;

Omar, Sanaa ;

Khairy, Heba .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 161 :1318-1328

[2] Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha:: formation of novel copolyesters in recombinant Escherichia coli [J].

Antonio, RV ;

Steinbüchel, A ;

Rehm, BHA .

FEMS MICROBIOLOGY LETTERS, 2000, 182 (01) :111-117

[3] Overexpression of succinyl-CoA synthase for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production in engineered Escherichia coli BL21(DE3) [J].

Bhatia, S. K. ;

Yi, D. -H. ;

Kim, H. -J. ;

Jeon, J. -M. ;

Kim, Y. -H. ;

Sathiyanarayanan, G. ;

Seo, H. M. ;

Lee, J. H. ;

Kim, J. -H. ;

Park, K. ;

Brigham, C. J. ;

Yang, Y. -H. .

JOURNAL OF APPLIED MICROBIOLOGY, 2015, 119 (03) :724-735

[4] Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) production from engineered Ralstonia eutropha using synthetic and anaerobically digested food waste derived volatile fatty acids [J].

Bhatia, Shashi Kant ;

Gurav, Ranjit ;

Choi, Tae-Rim ;

Jung, Hye-Rim ;

Yang, Soo-Yeon ;

Song, Hun-Suk ;

Jeon, Jong-Min ;

Kim, Jae-Seok ;

Lee, Yoo-Kyung ;

Yang, Yung-Hun .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 133 :1-10

[5] Fed-Batch mcl- Polyhydroxyalkanoates Production in Pseudomonas putida KT2440 and ΔphaZ Mutant on Biodiesel-Derived Crude Glycerol [J].

Borrero-de Acuna, Jose Manuel ;

Rohde, Manfred ;

Saldias, Cesar ;

Poblete-Castro, Ignacio .

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2021, 9

[6] Adaptation of Cupriavidus necator to conditions favoring polyhydroxyalkanoate production [J].

Cavalheiro, Joao M. B. T. ;

de Almeida, M. Catarina M. D. ;

da Fonseca, M. Manuela R. ;

de Carvalho, Carla C. C. R. .

JOURNAL OF BIOTECHNOLOGY, 2013, 164 (02) :309-317

[7] Fed-batch strategies using butyrate for high cell density cultivation of Pseudomonas putida and its use as a biocatalyst [J].

Cerrone, Federico ;

Duane, Gearoid ;

Casey, Eoin ;

Davis, Reeta ;

Belton, Ian ;

Kenny, Shane T. ;

Guzik, Maciej W. ;

Woods, Trevor ;

Babu, Ramesh P. ;

O'Connor, Kevin .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (22) :9217-9228

[8] Efficient production of polyhydroxyalkanoates (PHAs) from Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) as the sole carbon source [J].

Chanasit, Wankuson ;

Hodgson, Brian ;

Sudesh, Kumar ;

Umsakul, Kamontam .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2016, 80 (07) :1440-1450

[9] Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products [J].

Chee, J. -Y. ;

Lau, N. -S. ;

Samian, M. -R. ;

Tsuge, T. ;

Sudesh, K. .

JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (01) :45-54

[10] PHA synthase (PhaC): interpreting the functions of bioplastic-producing enzyme from a structural perspective [J].

Chek, Min Fey ;

Hiroe, Ayaka ;

Hakoshima, Toshio ;

Sudesh, Kumar ;

Taguchi, Seiichi .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (03) :1131-1141

← 1 2 3 4 5 6 7 8 9 10 →