Effect of astragaloside on cardiomyocyte apoptosis in murine coxsackievirus B3 myocarditis

被引：54

作者：

Zhang, Z.-C.

Li, S.-J.

Yang, Y.-Z.

Chen, R.-Z.

Ge, J.-B.

Chen, H.-Z.

机构：

[1] Fudan Univ, Zhongshan Hosp, Shanghai Inst Cardiovasc Dis, Minist Publ Hlth,Key Lab Viral Heart Dis, Shanghai 200032, Peoples R China

[2] Zhejiang Hosp, Hangzhou 310013, Zhenjiang Provi, Peoples R China

来源：

JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2007年 / 9卷 / 02期

基金：

中国国家自然科学基金;

关键词：

astragaloside; apoptosis; coxsackievirus B (CVB); myocarditis; microarray;

D O I：

10.1080/10286020412331286506

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Astragaloside is the major component of Astragalus membranaceus, one of the Chinese medical herbs, and has several pharmacological actions on cardiovascular system, including positive inotropic, antiarrhythmia and anti-oxidant activities. We have investigated the effect of astragaloside on cardiomyocyte apoptosis and expression of apoptosis-associated genes in mice with coxsackievirus B-3 (CVB3)-induced myocarditis, the former of which has been detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay and the latter determined by using a focused microarray. Results showed that cardiomyocyte apoptosis and expression of various apoptosis associated genes are inhibited after treatment with astragaloside. The anti-apoptotic activity of astragaloside may contribute to the improvement of clinical outcomes in treating myocarditis with pharmaceutics of Astragalus membranaceus.

引用

页码：145 / 151

页数：7

共 14 条

[1] MyD88, an adapter protein involved in interleukin-1 signaling [J].

Burns, K ;

Martinon, F ;

Esslinger, C ;

Pahl, H ;

Schneider, P ;

Bodmer, JL ;

Di Marco, F ;

French, L ;

Tschopp, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (20) :12203-12209

[2] An intact zinc ring finger is required for tumor necrosis factor receptor-associated factor-mediated nuclear factor-κB activation but is dispensable for c-Jun N-terminal kinase signaling [J].

Dadgostar, H ;

Cheng, GH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (38) :24775-24780

[3] Calpain inhibition protects against virus-induced apoptotic myocardial injury [J].

DeBiasi, RL ;

Edelstein, CL ;

Sherry, B ;

Tyler, KL .

JOURNAL OF VIROLOGY, 2001, 75 (01) :351-361

[4] Induction of cell death by tumour necrosis factor (TNF) receptor 2, CD40 and CD30:: a role for TNF-R1 activation by endogenous membrane-anchored TNF [J].

Grell, M ;

Zimmermann, G ;

Gottfried, E ;

Chen, CM ;

Grünwald, U ;

Huang, DCS ;

Lee, YHW ;

Dürkop, H ;

Engelmann, H ;

Scheurich, P ;

Wajant, H ;

Strasser, A .

EMBO JOURNAL, 1999, 18 (11) :3034-3043

[5] Naturoceutical agents in the management of cardiovascular disease [J].

Hermann D.D. .

American Journal of Cardiovascular Drugs, 2002, 2 (3) :173-196

[6] Coxsackievirus B3 induces apoptosis in the early phase of murine myocarditis: A comparative analysis of cardiovirulent and noncardiovirulent strains [J].

Joo, CH ;

Hong, HN ;

Kim, EO ;

Im, JO ;

Yoon, SY ;

Ye, JS ;

Moon, MS ;

Kim, D ;

Lee, H ;

Kim, YK .

INTERVIROLOGY, 2003, 46 (03) :135-140

[7] From myocarditis to cardiomyopathy: Mechanisms of inflammation and cell death - Learning from the past for the future [J].

Kawai, C .

CIRCULATION, 1999, 99 (08) :1091-1100

[8] TRAF1 is a substrate of caspases activated during tumor necrosis factor receptor-α-induced apoptosis [J].

Leo, E ;

Deveraux, QL ;

Buchholtz, C ;

Welsh, K ;

Matsuzawa, S ;

Stennicke, HR ;

Salvesen, GS ;

Reed, JC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :8087-8093

[9]

Miller A L, 1998, Altern Med Rev, V3, P422

[10] Mechanisms of apoptosis [J].

Reed, JC .

AMERICAN JOURNAL OF PATHOLOGY, 2000, 157 (05) :1415-1430

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