The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33

被引：45

作者：

Corratge-Faillie, Claire ^{[1
]}

Ronzier, Elsa ^{[1
,3
]}

Sanchez, Frederic ^{[1
]}

Prado, Karine ^{[1
,4
]}

Kim, Jeong-Hyeon ^{[1
]}

Lanciano, Sophie ^{[1
,5
]}

Leonhardt, Nathalie ^{[2
]}

Lacombe, Benoit ^{[1
]}

Xiong, Tou Cheu ^{[1
]}

机构：

[1] UM, INRA, UMR CNRS, Montpellier SupAgro,Biochim & Physiol Mol Plantes, Pl Viala, F-34060 Montpellier, France

[2] Univ Aix Marseille, Inst Biol Environm & Biotechnol, Lab Biol Dev Plantes,CNRS,CEA, Serv Biol Vegetale & Microbiol Environm,UMR7265, St Paul Les Durance, France

[3] Univ Rochester, Aab Cardiovasc Res Inst, 601 Elmwood Ave, Rochester, NY 14642 USA

[4] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland

[5] IRD, DIADE UMR232, 911 Ave Agropolis, Montpellier, France

来源：

FEBS LETTERS | 2017年 / 591卷 / 13期

关键词：

arabidopsis; CPKs; guard cells; oocytes; shaker K+ channels; voltage clamp; DEPENDENT PROTEIN-KINASES; OXYGEN SPECIES PRODUCTION; ACID SIGNAL-TRANSDUCTION; ABSCISIC-ACID; ANION CHANNEL; STOMATAL CLOSURE; K+ CHANNEL; CALCIUM; PHOSPHATASE; SLAC1;

D O I：

10.1002/1873-3468.12687

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca2+-dependent regulation of Shaker channels by Ca2+-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca2+-induced stomatal closure is impaired in two cpk33 mutant plants.

引用

页码：1982 / 1992

页数：11

共 51 条

[1] GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel [J].