Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

被引：15

作者：

Zhang, Xinhua ^{[1
]}

Teixeira da Silva, Jaime A. ^{[2
]}

Niu, Meiyun ^{[1
]}

Zhang, Ting ^{[1
]}

Liu, Huanfang ^{[3
]}

Zheng, Feng ^{[1
]}

Yuan, Yunfei ^{[1
]}

Li, Yuan ^{[1
]}

Fang, Lin ^{[1
]}

Zeng, Songjun ^{[1
]}

Ma, Guohua ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Im, South China Bot Garden, Guangzhou, Peoples R China

[2] POB 7,Miki Cho PO,Ikenobe 3011-2, Kita Dist, Kagawa 7610799, Japan

[3] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou, Peoples R China

来源：

PHYTOCHEMISTRY | 2021年 / 183卷

基金：

中国国家自然科学基金;

关键词：

Santalum album; Santalaceae; Nerolidol/linalool synthase; Stress response; TERPENE SYNTHASES; SESQUITERPENE SYNTHASES; LINALOOL BIOSYNTHESIS; MOLECULAR-CLONING; FLORAL SCENT; TRANSCRIPTOME; HEARTWOOD; PATHWAYS; IDENTIFICATION; (E)-NEROLIDOL;

D O I：

10.1016/j.phytochem.2020.112610

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Essential oils extracted from the heartwood of Indian sandalwood (Santalum album L.) contain linalool and nerolidol as minor components. However, nerolidol/linalool synthase (NES/LIS), which produce linalool and nerolidol, have yet to be characterized in sandalwood. Using a transcriptomic-based approach, a terpene synthase gene was screened from unigenes of transcriptome data derived from S. album seedlings exposed to low temperature (4 degrees C). The enzyme encoded by these complementary DNAs belongs to the TPS-b Glade. Recombinant SaNES/LIS is a bifunctional enzyme that can catalyze the formation of (E)-nerolidol from farnesyl diphosphate and linalool from geranyl diphosphate, respectively. Whereas SaNES/LIS was primarily localized in chloroplastids, both as granular fluorescence and as diffuse fluorescence, it was also detected in the cytosol of a limited number of cells. Agrobacterium tumefaciens-mediated transient gene expression in planta produced the same terpene products as those obtained in vitro. Real-time PCR analysis showed the highest expression of SaNES/LIS in fruits, with about a three-fold higher level than in leaves, followed by flowers, heartwood and roots. SaNES/LIS transcripts were differentially activated in different tissues in response to methyl jasmonate, cold, high temperature, strong illumination, and drought stress. Our results provide novel insight into the role of sandalwood terpenoids in response to various environmental stresses.

引用

页数：12

共 55 条

[1] Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia' [J].