Anti-laminin-1 antibodies in serum and follicular fluid of women with Hashimoto's thyroiditis undergoing in vitro fertilization

被引:13
作者
Caccavo, Domenico [1 ]
Pellegrino, Nelly M. [1 ]
Nardelli, Claudia [2 ]
Vergine, Silvia [2 ]
Leone, Luca [2 ]
Marolla, Alessandra [2 ]
Vacca, Margherita P. [2 ]
Depalo, Raffaella [2 ]
机构
[1] Univ Bari Aldo Moro, Univ Hosp, Immunol Sect, Dept Biomed Sci & Human Oncol, Piazza G Cesare 11, I-70124 Bari, Italy
[2] Univ Hosp, Dept Gen Surg Gynecol Obstet & Anesthesiol, Unit Pathophysiol Human Reprod & Gametes Cryopres, Bari, Italy
关键词
anti-laminin-1; Ab; Hashimoto's thyroiditis; in vitro fertilization (IVF) outcome; EMBRYO-TRANSFER; REPRODUCTIVE FAILURE; ANTITHYROID ANTIBODIES; MOUSE EMBRYO; PREGNANCY; AUTOIMMUNITY; AUTOANTIBODIES; ENDOMETRIOSIS; LAMININ; ABORTION;
D O I
10.1177/0394632015627281
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of this study is to evaluate the presence of anti-laminin-1 antibodies (aLN-1) in sera and follicular fluid (FF) of infertile women affected by Hashimoto's thyroiditis (HT) undergoing in vitro fertilization (IVF) and its impact on oocyte maturation and IVF outcome. aLN-1 were measured by a home-made enzyme linked immunosorbent assay (ELISA) in: (1) sera and FF from 44 infertile women affected by HT (HTIW) with tubal factor or male factor as primary cause of infertility; (2) in sera and FF from 28 infertile women without HT, with tubal factor or male factor as cause of infertility (infertile controls-ICTR); and (3) in sera from 50 fertile women (FW). aLN-1 serum levels were significantly higher in HTIW when compared with both fertile women and ICTR (P < 0.001and P < 0.01, respectively). Assuming as cutoff the 99th percentile of values obtained in sera of FW, 43.2% of HTIW and 3.6% of ICTR were aLN-1 positive (P = 0.0001). Also aLN-1 detected in FF from HTIW were significantly higher in comparison with those found in FF of ICTR (P = 0.006). In HTIW, metaphase II oocyte count showed inverse correlation with both serum and FF aLN-1 levels (r = 0.34, P = 0.02 and r = 0.33, P = 0.03, respectively). Implantation and pregnancy rates were significantly lower in HTIW (7.9% and 9.1%, respectively) when compared with ICTR (23% and 31.1%, respectively) (P = 0.015 and P = 0.03, respectively). Our results demonstrated for the first time the presence of aLN-1 in a relevant percentage of HTIW and suggest that these auto-antibodies may impair IVF outcome.
引用
收藏
页码:280 / 287
页数:8
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