Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

被引：250

作者：

Monostori, Peter ^{[1
]}

Wittmann, Gyula ^{[1
]}

Karg, Eszter ^{[1
]}

Turi, Sandor ^{[1
]}

机构：

[1] Univ Szeged, Dept Pediat, H-6720 Szeged, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 28期

基金：

美国国家科学基金会;

关键词：

Glutathione; Glutathione disulfide; Glutathione status; Oxidative stress; Redox homeostasis; Thiol status; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; RED-BLOOD-CELLS; INDUCED FLUORESCENCE DETECTION; CAPILLARY-ZONE-ELECTROPHORESIS; MOVING BOUNDARY ELECTROPHORESIS; DESORPTION/IONIZATION ION-TRAP; PROTEIN S-GLUTATHIONYLATION; ENZYMATIC RECYCLING METHOD; FLOW-INJECTION ANALYSIS;

D O I：

10.1016/j.jchromb.2009.06.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathione (GSH) is a thiol-containing tripeptide, which plays central roles in the defence against oxidative damage and in signaling pathways. Upon oxidation, GSH is transformed to glutathione disulfide (GSSG). The concentrations of GSH and GSSG and their molar ratio are indicators of cell functionality and oxidative stress. Assessment of redox homeostasis in various clinical states and medical applications for restoration of the glutathione status are of growing importance. This review is intended to provide a state-of-the-art overview of issues relating to sample pretreatment and choices for the separation and detection of GSH and GSSG. High-performance liquid chromatography, capillary electrophoresis and gas chromatography (as techniques with a separation step) with photometric, fluorimetric, electrochemical and mass spectrometric detection are discussed, stress being laid on novel approaches. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3331 / 3346

页数：16

共 200 条

[1] Reverse micellar microextraction for rapid analysis of thiol-containing peptides and amino acids by atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].