The effect of nitrogen (N) supply and weeds on grain yield of spring barley was investigated from 1997 to 2004 in an organic farming crop rotation experiment in Denmark on three different soil types varying from coarse sand to sandy loam. Two experimental factors were included in the experiment in a factorial design: (1) catch crop (with and without), and (2) manure (with and without). The crop rotation included grass-clover as a green manure crop. Animal manure was applied as slurry in rates corresponding to 40% of the N demand of the cereal crops. Application of 50 kg NH4-N ha(-1) in manure (slurry) increased average barley grain DM yield by 1.0-1.3 Mg DM ha(-1), whereas the use of catch crops (primarily perennial ryegrass) increased grain DM yield by 0.2-0.4 Mg DM ha(-1) with the smallest effect on the loamy sand and sandy loam soils and the greatest effect on the coarse sandy soil. Model estimations showed that the average yield reduction from weeds varied from 0.2 to 0.4 Mg DM ha(-1) depending on weed species and density. The yield effects of N supply were more predictable and less variable than the effects of weed infestation. The infestation level of leaf diseases was low and not a significant source of yield variation. The apparent recovery efficiency of N in grains (N use efficiency, NUE) from NH4-N in applied manure varied from 29 to 38%. The NUE of above-ground N in catch crops sampled in November prior to the spring barley varied from 16 to 52% with the largest value on the coarse sandy soil and the smallest value on the sandy loam soil. A comparison of grain yield levels obtained at the different locations with changes in soil organic matter indicated a NUE of 21-26% for soil N mineralisation, which is smaller than that for the mineral N applied in manure. However, this estimate is uncertain and further studies are needed to quantify differences in NUE from various sources of N. The proportion of perennial weeds in total biomass increased during the experiment, particularly in treatments without manure application. The results show that manure application is a key factor in maintaining good crop yields in arable organic fanning on sandy soils, and in securing crops that are sufficiently competitive against perennial weeds. (c) 2006 Elsevier B.V. All rights reserved.