Determination of eight penicillin antibiotics in pharmaceuticals, milk and porcine tissues by nano-liquid chromatography

This study describes the ability of nanoscale liquid chromatography (nano-LC) coupled with UV or mass spectrometry (MS) for the simultaneous determination of eight common penicillin antibiotics (amoxicillin, ampicillin, penicillin G, penicillin V, oxacillin, cloxacillin, nafcillin and dicloxacillin) in commercial samples (pharmaceuticals, milk, porcine tissues (liver and kidney)) for the first time. Material types of the on-column polymeric frits (polystyrene-based and polymethacrylate-based monoliths) and the packed stationary phase materials (C8 and C18 particles of 3 mu m) used in the nano-LC for the influence of penicillin separation were evaluated. The nano-LC and MS parameters such as the composition and flow rate of mobile phase, capillary voltage and temperature of dry gas were examined in order to acquire high separation resolution and detection sensitivity for penicillin analyses. Furthermore, a home-made in-line filter (a nylon membrane of 0.2 mu m pore size), was first used to connect with the flow cell of high sensitivity UV detector or the nanoelectrospray needle in MS detection. The result indicated it could effectively improve the reproducibility of penicillin mass signals or prolong the lifetime of the flow cell. The nano-LC methods provided good quantitative precisions in the range of 89.5-111.2% for UV detection at 0.5 mu g/mL penicillins, and 83.1-94.9% for MS detection at 5 mu g/L penicillins), respectively, as well as offered stable retention repeatabilities (the relative standard deviation (RSD) of retention time was lower 0.30% in both the UV and MS detections). Compared to other LC-MS methods, the proposed nano-LC systems provided better detection sensitivity for these penicillins (the limits of detection (LOD) was of 2.27-4.06 mu g/L for UV mode, and 0.01-0.51 mu g/L for MS mode) when either UV or MS detector was employed. (C) 2009 Elsevier B.V. All rights reserved.